中国医科大学学报2023，Vol.52Issue(12)：1062-1067,6.DOI:10.12007/j.issn.0258-4646.2023.12.002
PPAR-γ抑制高糖微环境下NCI-H460细胞增殖的分子机制
PPAR-γ inhibits the proliferation of human large cell lung cancer NCI-H460 cells through the NLRP3 inflammasome in a high-glucose microenvironment
摘要
Abstract
Objective To investigate the effect of PPAR-γon the proliferation of human large cell lung cancer(NCI-H460)cells in a high-glucose microenvironment and to explore the associated molecular mechanism.Methods NCI-H460 cells were treated with normal-glucose(blank group),hypertonic(control group),and high-glucose(30 mmol/L;high-glucose group)media.The effects of a high-glucose microenvironment on the proliferation of NCI-H460 cells were analyzed using Cell Counting Kit 8(CCK-8)and colony-for-mation assays.The specific PPAR-γactivator,rosiglitazone,was used to treat NCI-H460 cells in a high-glucose microenvironment,and the expression of NLRP3-inflammasome-related proteins was detected by Western blotting.The effect of PPAR-γon the proliferation of NCI-H460 cells in a high-glucose microenvironment was analyzed by CCK-8 and clony-formation assays.The NLRP3 inflammasome agonist,NSS,combined with rosiglitazone,were used to treat NCI-H460 cells in a high-glucose microenvironment.CCK-8 and clony-formation assays were used to analyze whether the NLRP3 inflammasome was involved in the inhibitory effect of PPAR-γon the proliferation of NCI-H460 cells in a high-glucose microenvironment.Results A high-glucose microenvironment significantly induced the proliferation of NCI-H460 cells,increased the activity of the NLRP3 inflammasome,and reduced the expression levels of PPAR-γprotein.Rosiglitazone effectively inhibited NLRP3 inflammasome activity and NCI-H460 cell proliferation,and this effect was reversed by NLRP3 inflammasome agonist.Conclusion PPAR-γinhibits the proliferation of NCI-H460 cells in a high-glucose microenvironment by down-regulating the activity of the NLRP3 inflammasome.关键词
PPAR-γ/NLRP3炎症小体/高糖微环境/增殖/人大细胞肺癌Key words
PPAR-γ/NLRP3 inflammasome/high-glucose microenvironment/proliferation/human large cell lung cancer分类
医药卫生引用本文复制引用
胡明亮..PPAR-γ抑制高糖微环境下NCI-H460细胞增殖的分子机制[J].中国医科大学学报,2023,52(12):1062-1067,6.基金项目
辽宁省自然科学基金(2019-ZD-0739) （2019-ZD-0739）
