中国药理学与毒理学杂志2023，Vol.37Issue(12)：951-958,8.DOI:10.3867/j.issn.1000-3002.2023.12.008
基于活性和基于亲和性的蛋白质表达谱在药理毒理学研究中的应用进展
Applications of activity-based protein profiling and affinity-based protein profiling in pharmacology and toxicology research
摘要
Abstract
Activity-based protein profiling(ABPP)and affinity-based protein profiling(AfBPP)are reliable chemical proteomics techniques that exhibit significant advantages in identifying the direct acting targets of small molecule drugs and toxicants,which can help researchers understand the pharmaco-logical and toxicological mechanisms of active small molecules.This paper introduces ABPP and AfBPP technologies and summarizes the applications of this technique in drug off-target effect and target iden-tification of toxicants in recent years,such as drug off-target effect of crenolanib,BIA 10-2474,orlistat and target identification of VX,fenitrothion,acrolein.It is hoped that this review will give readers a better idea of ABPP/AfBPP and offer a line of thinking for researchers in the fields of pharmacology,toxicology and chemical biology.关键词
基于活性的蛋白质表达谱/基于亲和性的蛋白质表达谱/靶标鉴定/药物脱靶效应/毒性化合物Key words
activity-based protein profiling/affinity-based protein profiling/target identification/drug off target/toxic compound分类
医药卫生引用本文复制引用
陈佳胜,张鑫月,刘俊宏,孟文琪,张玲..基于活性和基于亲和性的蛋白质表达谱在药理毒理学研究中的应用进展[J].中国药理学与毒理学杂志,2023,37(12):951-958,8.基金项目
国家自然科学基金(82271916) （82271916）
国家自然科学基金(81801869) National Natural Science Foundation of China(82271916) （81801869）
and National Natural Science Foundation of China(81801869) （81801869）
