中国油料作物学报2023，Vol.45Issue(6)：1109-1118,10.DOI:10.19802/j.issn.1007-9084.2023028
拟南芥AtTrx5增强植物菌核病的抗性研究
Arabidopsis thioredoxin gene(AtTrx5)enhances plant resistance to Sclerotinia
摘要
Abstract
Sclerotinia is a significant disease that harms the production of cruciferous crops.S.sclerotiorum can induce host to produce excessive reactive oxygen species(ROS),resulting in host cell death,and Trx plays an important role in host antioxidant process.Screening and identifying the relationship between Trx and Sclerotinia re-sistance will provide genetic resources for plant resistance breeding.In this study,a key gene AtTrx5 was selected from A.thaliana through bioinformatics and transcriptome data analysis.AtTrx5 has the closest relationship with Brassica napus BnaA08g04320D with 85%amino acid homology.Trx5 showed up-regulated expression in the ho-mologous gene in rape.The lesion area of the AtTrx5 overexpression lines was reduced by 30%-39%compared with the WT,while the lesion area of T-DNA mutant was increased by about 39.2%at 24 hpi with S.sclerotiorum.The expression level of AtTrx5 in the overexpression lines was higher than wide type,while AtTrx5 was lower in the T-DNA mutant during the infection.DAB staining,H2O2 content,·O2-content indicated that the accumulation of ROS in T-DNA mutants was significantly higher than that of wide type;while the accumulation of ROS was significantly less in overexpression lines.关键词
核盘菌/抗病性/拟南芥/硫氧还蛋白/活性氧Key words
Sclerotinia sclerotiorum/disease resistance/Arabidopsis/Trx/ROS(reactive oxygen species)分类
农业科技引用本文复制引用
闫宝琴,陈燕桂,史贤成,李娜,丁一娟,钱伟..拟南芥AtTrx5增强植物菌核病的抗性研究[J].中国油料作物学报,2023,45(6):1109-1118,10.基金项目
国家自然科学基金(31801395,31971978) （31801395,31971978）
重庆市基础研究与前沿探索专项(自然科学基金)面上项目(cstc2019jcyj-msxmX0486) （自然科学基金）
西南大学大学生创新创业训练计划(S202210635142) （S202210635142）
甘肃省青年科技基金(23JRRL0005) （23JRRL0005）
