中国油料作物学报2023，Vol.45Issue(6)：1119-1127,9.DOI:10.19802/j.issn.1007-9084.2023058
甘蓝型油菜-核盘菌酵母双杂交cDNA文库构建及BnJar1互作蛋白筛选
Construction of a Brassica napus-Sclerotinia sclerotiorum yeast two-hybrid cDNA library and screening for BnJar1 interacting proteins
摘要
Abstract
During the interaction between Brassica napus and Sclerotinia sclerotiorum,the expression of JA(jasmonic acid)synthesis-related genes increases,while JA signal transduction is inhibited.It is speculated that the effector proteins secreted by S.sclerotiorum may directly or indirectly interact with JAR1 protein,reducing its activi-ty and thus inhibiting the defense system of B.napus dependent on JA pathway.The full-length sequence of the dif-ferentially expressed gene BnJar1 was obtained by transcriptome sequencing in the early stage of this research.In order to further reveal the mechanism of BnJar1 gene involved in the interaction between B.napus and S.sclerotio-rum,this study analyzed and predicted the bioinformatics of BnJAR1 protein,constructed a yeast expression gene cDNA library of B.napus-S.sclerotiorum interaction,and used BnJar1 as bait protein to screen the library by yeast two-hybrid.Through the study,5 interacting proteins from B.napus were obtained:BnAOC2,BnCOP9,BnDcoH,BnNIT2,and BnTSJT1;and 2 interacting proteins from S.sclerotiorum:SsMFS and SsRho3.The relevant results contributed to our research on the interaction between B.napus and S.sclerotiorum,exploration of pathogenic or re-sistance-related genes,and on further elucidation of their mechanisms.关键词
甘蓝型油菜/核盘菌/酵母双杂交文库/BnJar1蛋白/互作蛋白Key words
Brassica napus.L/Sclerotinia sclerotiorum/yeast two-hybrid cDNA library/BnJar1 protein/in-teracting protein分类
农业科技引用本文复制引用
彭琦,王晓东,张洁夫,周晓婴,高建芹,张维,孙程明,胡茂龙,浦惠明,郭月,付三雄..甘蓝型油菜-核盘菌酵母双杂交cDNA文库构建及BnJar1互作蛋白筛选[J].中国油料作物学报,2023,45(6):1119-1127,9.基金项目
江苏省重点研发计划(BE2022340) （BE2022340）
江苏省自然科学基金(BK20191237) （BK20191237）
国家自然科学基金(31771834) （31771834）
现代农业产业技术体系建设专项资金(CARS-12) （CARS-12）
