中国油料作物学报2023，Vol.45Issue(6)：1128-1140,13.DOI:10.19802/j.issn.1007-9084.2023209
云南省芥菜型油菜菌核病抗性鉴定及抗性位点全基因组关联分析
Identification of resistance to Sclerotinia sclerotiorum and genome-wide association analysis of resistance sites in Brassica juncea from Yunnan Province
摘要
Abstract
Sclerotinia stem rot is a broad-spectrum threat to rapeseed production,and Brassica juncea contains abundant genetic resources for resistance against Sclerotinia sclerotiorum.Therefore,it is of great significance to car-ry out screening,localization,and exploration of resistance loci for sclerotinia stem rot in Brassica juncea germplasm resources for the breeding of Sclerotinia-resistant rapeseed.In this study,the S.sclerotiorum resistance of Brassica juncea germplasm resources collected from various regions in Yunnan Province was evaluated under three different growth environments,and a whole-genome association analysis of S.sclerotiorum resistance loci was conducted based on the resequencing data of 91 Brassica juncea germplasm materials.According to the results of leaf identifi-cation under in vitro conditions in the three growth environments,one highly resistant germplasm material J7667 and one relatively resistant germplasm material J047 were selected.The resequencing data of the 91 materials were aligned to the reference genome of Brassica juncea for variation detection analysis,resulting in the detection of 11 337 871 SNPs and 40 170 Indels distributed on the 18 chromosomes of Brassica juncea.After data filtering,403 211 high-quality SNPs were obtained.The phenotypic data of in vitro leaves,in vitro stems,and stem resistance to S.sclerotiorum of the 91 materials under the three growth environments were subjected to whole-genome associa-tion analysis using two analysis software,Emmax and Tassel.In the in vitro leaves,both analysis methods identified a total of 62 significantly associated SNP loci with resistance in the three different growth environments,including 23 and 39 significantly associated SNP loci in the Luoping winter sowing and Xiaoshao winter sowing environments,respectively,while no resistance loci were detected in the Xiaoshao summer sowing environment.In the stem of Luoping winter sowing,both methods detected 3 significantly associated SNP loci with resistance,while no signifi-cant SNP was detected in the stem of Xiaoshao summer sowing.In the Tassel method,closely located resistance SNP loci were detected on chromosomes A2,A8,and B2 in Luoping winter sowing and Xiaoshao winter sowing,while in the Emmax method,closely located resistance SNP loci were detected on chromosomes B5 and B8 in Luop-ing winter sowing and Xiaoshao winter sowing.In addition,this study mapped the previously identified S.sclerotio-rum resistance QTL loci and the resistance loci identified in this study to the reference genome of Brassica juncea.It was found that the resistance QTL intervals identified in this study(XLRA2-2,XLRA1-4)overlapped with the re-ported resistance QTL loci Sll2 and qsr10-1,respectively.关键词
芥菜型油菜/核盘菌/全基因组关联分析/抗性Key words
Brassica juncea/Sclerotinia sclerotiorum/genome-wide association analysis/resistance分类
农业科技引用本文复制引用
韩俣天,程小毛,俎峰,何晓莹,任静,张建昆,罗延青,陈苇,董云松,李庆刚,田志梅..云南省芥菜型油菜菌核病抗性鉴定及抗性位点全基因组关联分析[J].中国油料作物学报,2023,45(6):1128-1140,13.基金项目
云南省种子种业联合实验室项目(20205AR070001) （20205AR070001）
云南省创新人才培养对象项目(202305AD160025) （202305AD160025）
财政部和农业农村部:国家现代农业产业技术体系(CARS-12) （CARS-12）
兴滇英才支持计划青年人才项目(YNWR-QNBJ2019-280) （YNWR-QNBJ2019-280）
