中国油料作物学报2023，Vol.45Issue(6)：1150-1155,6.DOI:10.19802/j.issn.1007-9084.2023223
分子标记辅助选择创制抗根肿病油菜恢复系
Breeding of resistance to clubroot restore line in Brassica napus by molecular marker-assisted selection
摘要
Abstract
Currently,clubroot disease has become one of the main diseases of rapeseed in the Yangtze River basin,which seriously affects the development of rapeseed industry.To use molecular marker assisted selection technology to rapidly transfer parents with resistance to root nodules and high compatibility,18NS(carrying with Pb-Ba8.1 and CRb locus)was used as the donor parent for transferring resistant genes into the pol CMS restorer line 37R.Combined with molecular marker-assisted selection,comprehensive selection of field phenotypic traits,quali-ty analysis,combining ability determination,and resistance identification,a new parent 37RR with double resis-tance of brassica rapeseed to clubroot disease was developed.Through molecular marker-assisted selection,8 lines carrying PbBa8.1 + CRb locus were screened in BC3F3 generation.The results of field resistance identification of clubroot disease demonstrated that 8 lines showed immune resistance in Nanchong and Guangyuan.Combining abil-ity of 8 lines was 20S200-2>20S199-8>20S200-11>20S205-3>20S200-8>20S199-1>20S199-7>20S205-1 from high to low.The line 20S200-2 was finally identified and designated"37RR"in 2021.Its hybrid 1937R participated in the winter rape variety test in Southwest China,exhibited increased production at 8 points out of 9 test sites,with an average yield of 3204.00 kg·hm-2 per unit area.While gaining resistance to clubroot disease,its yield was also higher than that of the original hybrid Nanyou 1937.关键词
甘蓝型油菜/根肿病/PbBa8.1抗病位点/CRb抗病位点/分子标记辅助选择Key words
Brassica napus L./clubroot disease/PbBa8.1 resistance locus/CRb resistance locus/molecular marker assisted selection分类
农业科技引用本文复制引用
代兵兵,宋稀,余青青,杨玉恒,周锰林,赵昌斌,邓武明..分子标记辅助选择创制抗根肿病油菜恢复系[J].中国油料作物学报,2023,45(6):1150-1155,6.基金项目
四川省重大科技专项项目(2022ZDZX0015) （2022ZDZX0015）
国家油菜产业技术体系专项(CARS-12) （CARS-12）
