中国油料作物学报2023，Vol.45Issue(6)：1156-1165,10.DOI:10.19802/j.issn.1007-9084.2022330
甘蓝型油菜GLK基因家族全基因组鉴定及耐盐抗旱功能分析
Identification of GLK gene family and expression patterns under salt and drought stress in Brassica napus
摘要
Abstract
The GLK(the Golden2-like)transcription factor,a member of the GARP transcription factor super-family,can directly activate a large number of downstream target genes encoding photosynthesis-related proteins,including the target genes associated to chlorophyll biosynthesis,light capture and electron transport,and play a key role in plant physiological processes and abiotic stress responses.In order to identify GLK gene family in Brassica napus and explore their related functions in response to salt and drought stress,159 BnaGLK genes were identified on the whole genome level,and their gene structure characteristics,phylogenetic evolution,promoter cis-acting ele-ments and gene expression patterns were analyzed.The results showed that 159 BnaGLK genes were unevenly dis-tributed on 19 chromosomes.They were divided into 9 subgroups,and the number and structure of exons and in-trons of the BnaGLK gene were very similar in each subgroup.Promoter analysis revealed a number of cis-acting el-ements,including a variety of hormonal and abiotic stress responsive,particularly drought responsive cis-acting ele-ments.Eight BnaGLKs with high expression in leaves based on the open-accessed expression data,were selected for further expression pattern analysis under salt and drought stress by qRT-PCR.The results indicated that Bna-GLK genes play an important role in abiotic stress response in B.napus.This study laid a foundation for further analysis of the biological function of GLK gene family in B.napus.关键词
油菜/GLK基因家族/全基因组分析/干旱胁迫/盐胁迫/表达模式Key words
Brassica napus/GLK gene family/whole genome identification/drought stress/salt stress/expression分类
农业科技引用本文复制引用
邹雨婷,刘丹,王秋艳,夏柔,汪文倩,杨敏,童雅微,危文亮..甘蓝型油菜GLK基因家族全基因组鉴定及耐盐抗旱功能分析[J].中国油料作物学报,2023,45(6):1156-1165,10.基金项目
国家自然科学基金(31871661) （31871661）
