中国油料作物学报2023，Vol.45Issue(6)：1206-1216,11.DOI:10.19802/j.issn.1007-9084.2023216
油菜抗除草剂新种质PN19的交互抗性鉴定及其抗性基因的表达研究
Intercrossing-resistance of herbicide resistant novel germplasm PN19 in rapeseed and its expression of resistance genes
摘要
Abstract
The service life of herbicide-resistant varieties can be prolonged by identification of intercrossing-resistance of existing herbicide-resistant rapeseed and the screening of application of other safe herbicide in dosage forms.In this study,a new germplasm PN19 and transgenic plants of Arabidopsis and tobacco resistant to SU herbi-cides were used as materials.Five classes of ALS inhibitory herbicides with five concentration gradients were sprayed at 3-5 leaf stage of rapeseed.The intercrossing-resistance of PN19 to five herbicides was determined by re-sistance identification.Results showed that plant intercrossing-resistance against SU and SCT class was the stron-gest,followed by IMI and TP class,whereas that against PB class was the lowest.Evaluation of herbicide intercross-ing-resistance in transgenic plants and analysis of in vitro activity of tobacco ALS enzymes revealed this resistance function to various herbicides was resulted from the decreased sensitivity of target ALS enzymes to herbicides.In ad-dition,the expression of PN19 herbicide-resistance gene after treatment with five herbicides was higher than that of wild type.The study provides an opportunity by mixing use of ALS herbicides to integratedly control weeds in the field.关键词
油菜/ALS抑制类除草剂/交互抗性/基因表达/体外酶活Key words
rapeseed/ALS-inhibiting herbicides/cross resistance/gene expression/in vitro enzyme activity分类
农业科技引用本文复制引用
郭月,胡茂龙,卢鑫宇,王婷,李伟龙,祁余容,吕欣蕾,彭琦,高建芹,张洁夫..油菜抗除草剂新种质PN19的交互抗性鉴定及其抗性基因的表达研究[J].中国油料作物学报,2023,45(6):1206-1216,11.基金项目
国家自然科学基金青年科学基金(31901503) （31901503）
江苏省农业科技自主创新资金(CX(22)3084) （CX(22）
江苏省农业生物学重点实验室重大自主科研项目(JKLA2021-ZD02) （JKLA2021-ZD02）
江苏省自然科学基金青年基金(BK20190267) （BK20190267）
国家农业现代产业技术体系建设专项(CARS-12) （CARS-12）
