中国油料作物学报2023，Vol.45Issue(6)：1217-1226,10.DOI:10.19802/j.issn.1007-9084.2022224
甘蓝型油菜种子低温萌发差异及RNA-seq分析
Low-temperature stress caused phenotypic variation in germination and RNA-seq analysis for rapeseed(Brassica napus L.)
摘要
Abstract
For high efficient of rapeseed production by planting late-sowing cultivars in the lower reaches of the Yangtze River,material screening and mechanism of cold tolerance were carried out using 6 approved hybrid va-rieties and 6 temperature treatments were set.Seed germination characteristics and physiological indicators were studied under low temperature.RNA-seq technology was used for exploring germination differences under low tem-perature.Results showed that germination rate decreased under low temperature.Varieties Yueyou 1301 and Yuey-ou 1510 showed obvious advantages with respect to late sowing at low temperatures(<10℃).SOD activity were con-sistent with low temperature germination phenotype.Thus we identified 920 differentially-expressed genes(DGEs)that were involved in low temperature germination between Yueyou 1301 and Fengyou 737.Of these,21 DEGs were related to abscisic acid pathway and starch-sucrose metabolism pathway.In summary,Yueyou 1301 had strong low temperature germination ability.The difference in seed germination ability at low temperature might be caused by hormone signal transduction,activation of reactive oxygen species scavenging systems,and carbohydrate metabo-lism homeostasis,which might explain the strong cold tolerance of Yueyou 1301.关键词
甘蓝型油菜/低温胁迫/种子萌发/迟播/RNA测序Key words
Brassica napus L./low temperature stress/seed germination/late sowing/RNA-seq分类
农业科技引用本文复制引用
宋佳谕,蒋根水,陈宇眺,赵坚义,洪晓富..甘蓝型油菜种子低温萌发差异及RNA-seq分析[J].中国油料作物学报,2023,45(6):1217-1226,10.基金项目
杭州市种业公司合作水稻新种质创制(2021001) （2021001）
浙江省农业(旱粮新品种选育)新品种选育重大科技专项基金(2021C02064-2) （旱粮新品种选育）
浙江省数字旱粮重点实验室(2022E10012) （2022E10012）
