中国医学创新2023，Vol.20Issue(35)：24-29,6.DOI:10.3969/j.issn.1674-4985.2023.35.006
miR-25通过调节TGF-β信号传导通路促进胃癌细胞增殖和转移
miR-25 Promotes Proliferation and Transfer of Gastric Cancer Cell by Regulating TGF-β Signaling Pathway
摘要
Abstract
Objective:To explore the effects of miR-25 on proliferation,invasion,migration and apoptosis of human gastric cancer cell SGC-7901 and its molecular mechanism.Method:miR-25 interfering lentivirus was constructed,and the cells for study were divided into normal group,miR-25 interfering lentivirus group and miR-25 interfering lentivirus no-load group.Modern medical biology technology was used to detect transfection efficiency,cell proliferation ability,invasion ability,migration ability,apoptosis situation,and the expression of transforming growth factor-β(TGF-β)protein in each group.Result:Compared with the normal group,the expression of miR-25 in miR-25 interfering lentivirus group was significantly lower(P<0.05).Compared with the normal group,the survival rate,migration rate,proliferation rate and invasion ability of cell in miR-25 interfering lentivirus group were significantly decreased,and the apoptosis rate in miR-25 interfering lentivirus group was significantly higher,the differences were statistically significant(P<0.05).Compared with the normal group,the expression level of TGF-β protein in miR-25 interfering lentivirus group was increased,the difference was statistically significant(P<0.05).Conclusion:After the expression of miR-25 was inhibited in human gastric cancer cell SGC-7901,the expression of TGF-β protein in gastric cancer cell was significantly increased,and the invasion and metastasis ability of gastric cancer cell were significantly reduced,and the apoptosis of tumor cells was promoted.关键词
胃癌/miR-25/转化生长因子-β/信号通路Key words
Gastric cancer/miR-25/TGF-β/Signaling pathway引用本文复制引用
宋钉町,马博,曹博威,来比江·吾斯曼,张文斌..miR-25通过调节TGF-β信号传导通路促进胃癌细胞增殖和转移[J].中国医学创新,2023,20(35):24-29,6.基金项目
新疆维吾尔自治区自然科学基金计划基金项目(2021D01C372) （2021D01C372）
