中国肿瘤生物治疗杂志2023，Vol.30Issue(11)：950-956,7.DOI:10.3872/j.issn.1007-385x.2023.11.003
小鼠衰老所致"正虚"过程中免疫功能衰退表征的特点
Characteristic of immune function decline in the process of"Declined of Vital Qi"caused by aging in mice
摘要
Abstract
Objective:To investigate the characteristics of"Declined of Vital Qi"in the characterization of immune function decline caused by aging based on a mouse model of progressive aging.Methods:C57BL/6 mice of different months(2,6 and 15 months)were used to detect and compare the specific abundance of T cells,myeloid-derived suppressor cells(MDSC)and their subpopulations in peripheral blood and spleen of mice by flow cytometry.Results:In the peripheral blood,T cell subsets CD3+CD4+CD44-CD62L+ naïve CD4+ T cells(2 vs 6 months old,P=0.137;2 vs 15 months old,P=0.004;6 vs 15 months old,P=0.105)and CD3+CD8+CD44-CD62L+ naïve CD8+ T cells(2 vs 6 months old,P=0.179;2 vs 15 months old,P=0.001;6 vs 15 months old,P=0.015)showed a decrease in proportion to aging.However,the proportion of CD3+CD4+CD44+CD62L+ central memory CD8+ T cells increased with aging(2 vs 6 months old,P=0.01;2 vs 15 months old,P=0.007;6 vs 15 months old,P=0.164).The results in spleen experiment showed the same trend.At the same time,the proportion of CD8+ T cells increased gradually with aging(2 vs 6 months old,P=0.027;2 vs 15 months old,P<0.001;6 vs 15 months old.P<0.001).The subsets of activated CD8+ T cells with phenotype CD8+CD28+ also increased with age(2 vs 6 months old,P=0.863;2 vs 15 months old,P=0.016;6 vs 15 months old,P=0.024).Conclusion:In the process of"Declined of Vital Qi"caused by aging,the changes of different immune cell subsets do not all reflect the characteristics of immunosuppression,although the overall immune function decreases.A single phenotype is difficult to reflect the change of overall immune function.关键词
衰老/免疫功能/正虚/肺癌/T细胞/髓源性抑制细胞Key words
aging/immune function/Declined of Vital Qi/lung cancer/T cell/myeloid-derived suppressor cell(MDSC)分类
医药卫生引用本文复制引用
田建辉,姚望,阙祖俊,于盼,姚嘉良,罗斌..小鼠衰老所致"正虚"过程中免疫功能衰退表征的特点[J].中国肿瘤生物治疗杂志,2023,30(11):950-956,7.基金项目
国家自然科学基金(No.81774166) （No.81774166）
上海市卫生健康委领军人才基金(No.2022LJ014) （No.2022LJ014）
上海市炎癌转化病证生物学前沿研究基地课题(No.2021科技03-12) （No.2021科技03-12）
