中国肿瘤生物治疗杂志2023，Vol.30Issue(11)：965-972,8.DOI:10.3872/j.issn.1007-385x.2023.11.005
山柰酚通过Met/PI3K/Akt/mTOR通路诱导非小细胞肺癌NCI-H1650细胞发生自噬进而影响其增殖
Kaempferol promotes cell autophagy and affects cell proliferation of non-small cell lung cancer H1650 cells via regulating Met/PI3K/Akt/mTOR pathway
摘要
Abstract
Objective:To explore the specific effect and mechanism of kaempferol on inducing autophagy in non-small cell lung cancer(NSCLC)NCI-H1650 cells.Methods:Human NSCLC cell line NCI-H1650 was cultured and treated with kaempferol at different concentrations.The effects of kaempferol on NSCLC cell viability and proliferation were observed by CCK-8,MTT and EdU methods.LC3 adenovirus infection experiment was performed to investigate the effect of kaempferol on NSCLC cell autophagy.Western blot was used to detect the expression of key proteins of cell autophagy and relevant molecules of Met/PI3K/Akt/mTOR signaling pathway.Meanwhile,qPCR was used to detect the mRNA expression of Met in NCI-H1650 cells after kaempferol treatment.The transplanted tumor model of nude mice was established by using luciferase labeled A549-luc cells.The tumor growth was observed by in vivo animal imaging,and the expression of autophagy related key proteins and molecules of Met/PI3K/Akt/mTOR signaling pathway in the xenograft tissues were detected by Western blot.Results:Kaempferol significantly inhibited the proliferation of NCI-H1650 cells(P<0.05).After kaempferol treatment,the number of autophagosomes in NCI-H1650 cells was significantly increased(P<0.05),the expressions of autophagy related key proteins,LC3B and beclin1,were significantly increased(all P<0.05),and the expression of P62 was significantly decreased(P<0.05).Kaempferol significantly inhibited the mRNA and protein expression of Met,and inhibited the protein expression of p-PI3K p85,PI3K p85,p-Akt and p-mTOR(all P<0.05).Kaempferol inhibited the growth of transplanted tumor(P<0.05)and affected autophagy and the expression of Met/PI3K/Akt/mTOR pathway-related proteins in nude mouse tumor models(all P<0.05).Conclusion:Kaempferol induces autophagy in NSCLC NCI-H1650 cells by affecting the Met/PI3K/Akt/mTOR pathway,which in turn inhibites their proliferative capacity.关键词
山柰酚/非小细胞肺癌/NCI-H1650细胞/A549细胞/细胞自噬/Met/PI3K/Akt/mTOR通路/增殖Key words
kaempferol/non-small cell lung cancer(NSCLC)/NCI-H1650 cell/A549 cell/cell autophagy/Met/PI3K/Akt/mTOR pathway/proliferation分类
医药卫生引用本文复制引用
朱志明,王苏美,周绮纯,王娟娟,邓芷茵,唐青,吴万垠,王晰,万信良,莫瀚丹..山柰酚通过Met/PI3K/Akt/mTOR通路诱导非小细胞肺癌NCI-H1650细胞发生自噬进而影响其增殖[J].中国肿瘤生物治疗杂志,2023,30(11):965-972,8.基金项目
广东省中医药局面上研究项目(No.20222085) （No.20222085）
广东省教育厅研究项目(No.2020KTSCX029) （No.2020KTSCX029）
广东省中医证候临床研究开放课题(No.ZH2020KF03) （No.ZH2020KF03）
省部共建中医湿证国家重点实验室项目(No.SZ2021ZZ38) （No.SZ2021ZZ38）
