中国肿瘤生物治疗杂志2023，Vol.30Issue(11)：1009-1015,7.DOI:10.3872/j.issn.1007-385x.2023.11.011
结直肠癌组织和细胞中DEAD盒解旋酶10和Bystin样蛋白的表达及其临床意义
Expression of DEAD box RNA helicases 10 and Bystin-like proteins in colorectal cancer tissues and cells and their clinical significance
摘要
Abstract
Objective:To investigate the correlation between DEAD box RNA helicases 10(DDX10)and Bystin-like(BYSL)in colorectal cancer(CRC)tissues and colon cancer DLD-1 cells and its clinical significance.Methods:A total of 78 pairs of CRC tissues and corresponding paracancerous tissues surgically resected in the Second Affiliated Hospital of Fujian Medical University from March 2017 to March 2018 were collected for this study.The expression levels of DDX10 and BYSL in the cancer and paracancerous tissues were detected by immunohistochemistry EnVision method.SiDDX10 and siNC were transfected into DLD-1 cells by transient transfection technology,respectively.The effect of DDX10 expression on BYSL in colorectal cancer cells was detected by PCR.CCK-8 and transwell assays were used to verify the effect of DDX10 and BYSL on proliferation,migration and invasion of colorectal cancer cells.Results:The immunohistochemistry EnVision results showed that the expression levels of DDX10(73.08%)and BYSL(74.36%)were significantly higher in colorectal cancer tissues compared with paracancerous tissues.The results of chi-square test showed that the differences in DDX10 and BYSL expression were statistically significant in CRC patients with different tumor stages and different lymph node metastasis and recurrence status(all P<0.05).Pearson correlation analysis showed that DDX10 expression waspositively correlated with BYSL expression(r=0.636,P<0.001).Kaplan-Meier survival analysis showed that the high expression of DDX10 and BYSL was correlated with a worse prognosis of patients.Logistic regression analysis proved that DDX10 and BYSL were independent risk factors for CRC recurrence.The results of CCK-8 assay and Transwell assay showed that suppressing the expression of DDX10 could decrease the expression of BYSL and inhibit the proliferation,migration and invasion of colorectal cancer cells(all P<0.05).Conclusion:DDX10 and BYSL are highly expressed in CRC tissues,and they are positively associated with each other.Higher expression levels of DDX10 and BYSL are associated with poorer patient prognosis and were independent risk factors for CRC recurrence.Suppressing the expression of DDX10 can decrease the expression of BYSL,and inhibit the proliferation,migration and invasion of colorectal cancer cells.关键词
结直肠癌/DEAD盒解旋酶10/Bystin-like/复发/预后Key words
colorectal cancer(CRC)/DEAD box RNA helicases 10(DDX10)/Bystin-like(BYSL)/recurrence/prognosis分类
医药卫生引用本文复制引用
涂艳娟,郑雪琴,林欣怡,杨静波,邱燕如,许天文..结直肠癌组织和细胞中DEAD盒解旋酶10和Bystin样蛋白的表达及其临床意义[J].中国肿瘤生物治疗杂志,2023,30(11):1009-1015,7.基金项目
吴阶平医学基金(No.320.6750.2021-02-136) （No.320.6750.2021-02-136）
