中成药2023，Vol.45Issue(12)：3890-3894,5.DOI:10.3969/j.issn.1001-1528.2023.12.005
UPLC-MS/MS法同时测定朱日亨滴丸中15种成分
Simultaneous determination of fifteen constituents in Zhuriheng Dropping Pills by UPLC-MS/MS
摘要
Abstract
AIM To establish a UPLC-MS/MS method for the simultaneous content determination of ellagic acid,gallic acid,dehydrodiisoeugenol,methyleugenol,agarotetrol,elemicin,chlorogenic acid,geniposide,rutin,ferulic acid,hydroysafflor-yellow A,deoxycholic acid,cholic acid,borneol and taurine in Zhuriheng Dropping Pills.METHODS The analysis of 50%methanol solution of this drug was performed on a 30℃thermostatic Thermo C18 column(2.1 mm×100 mm,1.9 μm),with the mobile phase comprising of acetonitrile-0.1%formic acid(containing 10 mmol/L ammonium acetate)flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning.RESULTS Fifteen constituents showed good linear relationships within their own ranges(R2>0.990 0),whose average recoveries were 75.91%-112.13%with the RSDs of 3.06%-10.66%.CONCLUSION This simple,sensitive and efficient method can be used for the quality control of Zhuriheng Dropping Pills.关键词
朱日亨滴丸/化学成分/UPLC-MS/MSKey words
Zhuriheng Dripping Pills/chemical constituents/UPLC-MS/MS分类
药学引用本文复制引用
布仁,张彭鹏,王跃武,祖文,梁方圆,王小宁,陆景坤..UPLC-MS/MS法同时测定朱日亨滴丸中15种成分[J].中成药,2023,45(12):3890-3894,5.基金项目
国家自然科学基金(81960757) （81960757）
内蒙古科技成果转化项目(2019CG042) （2019CG042）
内蒙古自然科学基金(2019MS08119,2020MS08045) （2019MS08119,2020MS08045）
内蒙古医科大学百万工程项目(YKD2021MS033) （YKD2021MS033）
