中成药2023，Vol.45Issue(12)：3895-3901,7.DOI:10.3969/j.issn.1001-1528.2023.12.006
强心合剂HPLC指纹图谱建立及11种成分测定
Establishment of HPLC fingerprints and determination of eleven constituents for Qiangxin Mixture
摘要
Abstract
AIM To establish the HPLC fingerprints for Qiangxin Mixture,and to determine the contents of quercetin-3-O-β-D glucose 7-O-β-D gentil disglycoside(QGG),sinarin thiocyanate,erucinic acid,macrocetin,calycosin-7-glucoside,plananthine D,calycosin,baicalin,pilonidin,atracolactone Ⅲ and methylophiopogon dihydrogen isoflavone B.METHODS The analysis of methanol extract of this drug was performed on a 40℃thermostatic Agilent Eclipse XDB-C18 column(4.6 mm×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.01 mol/L phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 230 nm.RESULTS There were twenty common peaks in the fingerprints for fifteen batches of samples with the similarities of more than 0.930.Eleven constituents showed good linear relationships within their own ranges(r≥0.999 1),whose average recoveries were 98.47%-102.85%with the RSDs of 0.76%-1.74%.CONCLUSION This accurate and stable method can be used for the quality control of Qiangxin Mixture.关键词
强心合剂/HPLC指纹图谱/化学成分Key words
Qiangxin Mixture/HPLC fingerprints/chemical constituents分类
医药卫生引用本文复制引用
高紫薇,吴磊,林欣荣,陈晓虎,蒲维娅..强心合剂HPLC指纹图谱建立及11种成分测定[J].中成药,2023,45(12):3895-3901,7.基金项目
国家自然科学基金面上项目(81973824) （81973824）
江苏省研究生科研与实践创新计划项目(SJCX230757) （SJCX230757）
江苏省中医药管理局项目(YB2020014) （YB2020014）
江苏省中医院院内制剂研发项目(Y2022zj05) （Y2022zj05）
