中成药2023，Vol.45Issue(12)：3901-3905,5.DOI:10.3969/j.issn.1001-1528.2023.12.007
HPLC-MS/MS法同时测定荆防颗粒中10种成分
Simultaneous determination of ten constituents in Jingfang Granules by HPLC-MS/MS
郑振秋 1巩长芹 1张雪 1孙倩 1官玉静1
作者信息
- 1. 临沂市检验检测中心,山东 临沂 276000
- 折叠
摘要
Abstract
AIM To establish an HPLC-MS/MS method for the simultaneous content determination of pulegone,prim-O-glucosylcimifugin,5-O-methylvisammioside,columbianadin,saikosaponin a,saikosaponin d,ferulic acid,naringin,liquiritin and glycyrrhizic acid in Jingfang Granules.METHODS The analysis of methanol extract of this drug was performed on a 30℃thermostatic ZORBAX Eclipse Plus C18 column(2.1 mm×150 mm,1.8 μm),with the mobile phase comprising of methanol-0.1%formic acid flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning with multiple reaction monitoring mode.RESULTS Ten constituents showed good linear relationships within their own ranges(r>0.996 5),whose average recoveries were 96.7%-98.8%with the RSDs of 0.9%-1.9%.CONCLUSION This rapid,simple,sensitive and specific method can be used for the quality control of Jingfang Granules.关键词
荆防颗粒/化学成分/HPLC-MS/MSKey words
Jingfang Granules/chemical constituents/HPLC-MS/MS分类
医药卫生引用本文复制引用
郑振秋,巩长芹,张雪,孙倩,官玉静..HPLC-MS/MS法同时测定荆防颗粒中10种成分[J].中成药,2023,45(12):3901-3905,5.
