中成药2023，Vol.45Issue(12)：4011-4017,7.DOI:10.3969/j.issn.1001-1528.2023.12.026
化风丹药母发酵过程中优势菌种分离鉴定及不同温度和pH值下菌株生长情况
Isolation and identification of dominant strains in the fermentation process of HuaFengDan YaoMu and investigation of their growth under different temperature and pH value
摘要
Abstract
AIM To isolate and identify the dominant strains in the fermentation process of the HuaFengDan YaoMu,and to investigate the effects of different temperatures and pH values upon their growth.METHODS During the 0-6 weeks fermentation process of HuaFengDan YaoMu in the selective medium,the cultured bacteria,the molds and the yeasts had the colonies counted,and the dominant strains isolated and purified,particularly the dominant bacteria and fungi determined by 16S rDNA and 26S rDNA sequences.The optimal growth temperature and pH value of Bacillus aryabhattai,Debaryomyces hansenii,Aspergillus oryzae were investigated respectively.RESULTS HuaFengDan YaoMu found its bacterial counts increased from 0 to 4 weeks and stabilized from 4 to 6 weeks;its yeast counts increased during 0-3 weeks,and decreased continuously during 4-6 weeks;and its mold counts remained unchanged at 0 week,but increased steadily from 1 to 4 weeks,and decreased at 5 to 6 weeks of the fermentation.In the fermentation process of HuaFengDan YaoMu,the dominant strains of Bacillus aryabhattai,Debaryomyces hansenii and Aspergillus oryzae,identified and screened out by the phylogenetic tree,adapted best to the growth temperature of 35℃,25-30℃and 35℃,at pH value of 8,6 and 7,respectively.CONCLUSION In the fermentation process of HuaFengDan YaoMu,the dominant microorganisms of Bacillus aryabhattai,Debaryomyces hansenii,Aspergillus oryzae are identifiable and separable.关键词
化风丹药母/分离与鉴定/阿氏芽孢杆菌/汉逊德巴利酵母菌/米曲霉Key words
HuaFengDan YaoMu/isolation and identification/Bacillus aryabhattai/Debaryomyces hansenii/Aspergillus oryzae分类
医药卫生引用本文复制引用
曹国琼,刘敏,刘耀,徐剑,陈有丽,吴静澜,张永萍..化风丹药母发酵过程中优势菌种分离鉴定及不同温度和pH值下菌株生长情况[J].中成药,2023,45(12):4011-4017,7.基金项目
国家自然科学基金(81860704,82360774) （81860704,82360774）
贵州省科学技术厅科学技术基金(黔科合基础-ZK[2021]一般517) （黔科合基础-ZK[2021]一般517）
贵州省高等学校中药民族药(苗药)新剂型新制剂工程研究中心(黔教技[2022]022号) （苗药）
贵州省高层次创新型人才培养计划("千"层次)(2020) （"千"层次）
