中兽医医药杂志2023，Vol.42Issue(6)：12-15,4.DOI:10.13823/j.cnki.jtcvm.2023.111
5种中药水提物对大肠杆菌体外生物被膜形成的影响
Effect of five traditional Chinese medicines water extracts on biofilm formation of Escherichia coli
摘要
Abstract
To explore the effects of five Chinese herbal water extracts of Cortex Phellodendri,Fructus Forsythiae,Galla Chinensis,Radix Pulsatillae and Herba Taraxaci on the biofilm formation of Escherichia coli.The MIC of five Chinese herbal water extracts on Escherichia coli were determined by tube double dilution method,the effects of five Chinese herbal water extracts on the adhesion of Escherichia coli biofilm were observed by silver staining method,and the crystal violet staining method was used to detect the inhibitory effects of five Chinese herbal water extracts on the biofilm of Escherichia coli.The results showed that the MIC values of the water extracts of Cortex Phellodendri,Fructus Forsythiae,Galla Chinensis,Radix Pulsatillae and Herba Taraxaci were 500 mg/mL,500 mg/mL,250 mg/mL,250 mg/mL and 125 mg/mL against Escherichia coli,respectively.It could be observed in the mirror image that a large number of areas of dark spots were visible in the control group,while the area of dark spots were significantly reduced in the five medication groups.The OD value of Escherichia coli biofilm eluate in the control group was 0.555,while the OD values of eluate in the medication groups of Cortex Phellodendri,Fructus Forsythiae,Galla Chinensis,Radix Pulsatillae and Herba Taraxaci were 0.245,0.236,0.237,0.213 and 0.229,respectively,which were highly significant(P＜0.01)compared with the control group,and the inhibition rates of the five Chinese herbal water extracts on Escherichia coli biofilm were 55.9％,57.3％,57.5％,61.6％and 58.7％,respectively.Thus,all the five Chinese herbal water extracts were effective in inhibiting the formation of Escherichia coli biofilm.关键词
中药水提物/细菌生物被膜/大肠杆菌Key words
traditional Chinese medicines water extracts/bacterial biofilm/Escherichia coli分类
农业科技引用本文复制引用
王巧玉,甄明哲,张年洁,吴晓航,郭薪淼,王新..5种中药水提物对大肠杆菌体外生物被膜形成的影响[J].中兽医医药杂志,2023,42(6):12-15,4.基金项目
黑龙江省农垦总局"十三五"重点科技计划项目(HNK135-04-03) （HNK135-04-03）
