中兽医医药杂志2023，Vol.42Issue(6)：16-20,5.DOI:10.13823/j.cnki.jtcvm.2023.099
五倍子颗粒质量标准研究
Study on the quality standard of Galla Chinensis granules
摘要
Abstract
To establish a quality control standard for Galla Chinensis granules.Thin layer chromatography(TLC)was used to qualitatively identify Galla Chinensis medicinal materials,and gallic acid content was determined by high performance liquid chromatography(HPLC),the column was Waters Xselect® HSS T3(250.0 mm×4.6 mm,5 μm),the mobile phase was acetonitrile-formic acid solution(pH 3.2,gradient elute),the flow rate was 0.5 mL/min,the detection wavelength was 273 nm,and the column temperature was 30℃.Additionally,the particle size,moisture,solubility and loading capacity of Galla Chinensis granules were checked.The results showed that galic acid had a good linear relationship with the peak area in the range of 16.05-256.80 μg(R2=0.999 9).The RSD of precision test,stability test and repeatability test was not more than 1.0％;the average dosing recovery rate was 95.83％with RSD of 1.29％.The particle size,moisture,solubility and loading volume were in line with the provisions under granules in the 2020 edition of Chinese Veterinary Pharmacopoeia.The presented result suggested TLC and HPLC methods had strong specificity,good repeatability,accuracy and reliability,and could effectively evaluate the quality of Galla Chinensis granules.关键词
五倍子颗粒/质量标准/薄层鉴别/没食子酸/高效液相色谱Key words
Galla Chinensis granules/quality standard/TLC/gallic acid/HPLC分类
农业科技引用本文复制引用
吴雪琴,茹梦珂,贺泂杰,王玲,郑笑红,张长安,王胜义,崔东安..五倍子颗粒质量标准研究[J].中兽医医药杂志,2023,42(6):16-20,5.基金项目
中央级公益性科研院所基本科研业务费专项(Y2022YJ14) （Y2022YJ14）
兰州市科技计划项目(2022-2-61) （2022-2-61）
湖北省揭榜制科技项目(2022BEC033) （2022BEC033）
兽用天然药物与抗生素替代创新工程项目(25-LZIHPS-03) （25-LZIHPS-03）
