中国现代医生2023，Vol.61Issue(36)：1-4,67,5.DOI:10.3969/j.issn.1673-9701.2023.36.001
miR-29a影响eNOS蛋白表达参与血管内皮障碍机制研究
Mechanism of miR-29a affecting eNOS protein expression and participating in vascular endothelial dysfunction
摘要
Abstract
Objective To explore the inhibitory effect of miR-29a on in vitro angiogenesis of vascular endothelial cells by targeting cells with glutaminase,and preliminarily explore the relevant mechanisms of inflammatory factors inducing vascular endothelial dysfunction.Methods Human umbilical vein endothelial cells were selected as research cell lines,and the cell lines were intervened by recombinant human tumor necrosis factor-α(TNF-α)and serum amyloid A(SAA)respectively.The expression changes of miRNA were detected by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).The expression changes of endothelial nitric oxide synthase(eNOS)were detected by Western blot.Finally,the vascular protective drug salvia miltiorrhiza was used to reverse verify the expression changes of miR-29a and eNOS.Results The addition of TNF-α and SAA significantly increased the expression level of miR-29a compared with control group(P<0.05).qRT-PCR showed that the higher the level of inflammatory factors,the higher the expression level of cell line miR-29a.With 1.0ng/ml TNF-α and SAA as a constant dose,it was found that the expression level of miR-29a increased gradually with time.Western blot showed that the addition of TNF-α and SAA would decrease the level of eNOS protein in the cell line.The expression level of miR-29a decreased with the increase of salvia miltiorrhiza concentration,and decreased with time at constant salvia miltiorrhiza concentration.After adding salvia miltiorrhiza,the expression of eNOS protein increased obviously.Conclusion Inflammatory factors TNF-α and SAA can participate in the process of vascular endothelial injury by inducing the expression of miR-29a,and eNOS protein is widely involved in this process.The application of vasoprotective drugs can inhibit the over-expression of miR-29a to some extent,reduce the expression of eNOS protein,and thus play a role in protecting blood vessels.关键词
炎症因子/肿瘤坏死因子-α/血清淀粉样蛋白A/miR-29a/内皮型一氧化氮合酶Key words
Inflammatory factors/Tumor necrosis factor-α/Serum amyloid protein A/miR-29a/Endothelial nitric oxide synthase分类
临床医学引用本文复制引用
邹鹏涛,何磊,张方,朱波..miR-29a影响eNOS蛋白表达参与血管内皮障碍机制研究[J].中国现代医生,2023,61(36):1-4,67,5.基金项目
江西省卫生计生委科技计划项目(20195199) （20195199）
