中药材2023，Vol.46Issue(4)：852-856,5.DOI:10.13863/j.issn1001-4454.2023.04.010
西藏地区紫堇属植物DNA条形码鉴定
Identification of DNA Barcoding of Corydalis Plants in Tibet
摘要
Abstract
Objective:To use ITS2 sequence and psbA-trnH sequence as DNA barcodes for molecular identification of Corydalis plants from Tibet.Methods:By comparing the amplification success rate and sequencing success rate of the two sequences,the sequence characteristics were analyzed,the intraspecic and interspecific genetic distances were calculated.Neighbor-joining evolutionary tree was constructed to evaluate the identification efficiency of the two sequences.Results:The success rate of amplification and sequencing of the two sequences was 100％.The maximum intraspecific genetic distance of psbA-trnH sequence was smaller than the minimum inter-specific genetic distance,which met the genetic distance requirement of DNA barcodes.In phylogenetic tree analysis,psbA-trnH se-quence could effectively distinguish different Corydalis plants,but ITS2 sequence had poor clustering effect.Conclusion:psbA-trnH se-quence has high identification efficiency and can be used as DNA barcodes for the identification of Corydalis plants in Tibet.The study lays a solid foundation for the further study on the genomics of Corydalis plants in Tibet and provides a new method for the quality con-trol of Tibetan medicine of Corydalis plants in Tibet.关键词
紫堇属植物/DNA条形码/ITS2/psbA-trnHKey words
Corydalis plants/DNA barcode/ITS2/psbA-trnH分类
医药卫生引用本文复制引用
杨冬璠,赵丽萍,汪圣,旦增顿珠,徐福春..西藏地区紫堇属植物DNA条形码鉴定[J].中药材,2023,46(4):852-856,5.基金项目
西藏自治区科技厅重点研发与转化计划项目(XZ201901-GB-11) （XZ201901-GB-11）
西藏自治区自然科学基金项目(XZ2018 ZR G-134) （XZ2018 ZR G-134）
