中药材2023，Vol.46Issue(4)：892-902,11.DOI:10.13863/j.issn1001-4454.2023.04.018
基于UPLC-Q-TOF-MS技术的紫丁香化学成分及入血成分研究
Study on the Chemical Constituents and Components into Blood of Syringa oblata Based on UPLC-Q-TOF-MS
摘要
Abstract
Objective:To study on the chemical constituents of Syringa oblata and the components into blood after intragastric ad-ministration of Syringa oblata in rats.Methods:UPLC-Q-TOF-MS was performed on a Welch XB-C18 column(100 mmx2.1 mm,1.8μm),the mobile phase was 0.1％formic acid solution-acetonitrile for gradient elution,the flow rate was 0.3 mL/min,column tempera-ture was 40 ℃,injection volume was 2 μL.The chemical components and components into blood of Syringa oblata were characterized by comparison of reference materials,literature data and MassBank database information.Results:Thirty-eight chemical constituents were found from the extract of Syringa oblata,including lignans and sesquiterpenes;40 components were found from rat serum after intragas-tric administration,including 14 prototype components and 26 metabolites.Conclusion:In this study,UPLC-Q-TOF-MS technology and serum pharmacochemical method of traditional Chinese medicine are used to rapidly analyze the chemical components and components into blood of Syringa oblata,and preliminarily explore the possible pharmacodynamic substance basis of Syringa oblata,which provide a reference for further development and utilization of Syringa oblata.关键词
紫丁香/UPLC-Q-TOF-MS/化学成分/入血成分Key words
Syringa oblata Lindl./UPLC-Q-TOF-MS/Chemical constituents/Components into blood分类
医药卫生引用本文复制引用
蔡定吉,李志强,彭万钱,张皓男,何明珍,冯育林,钟国跃..基于UPLC-Q-TOF-MS技术的紫丁香化学成分及入血成分研究[J].中药材,2023,46(4):892-902,11.基金项目
国家重点研发计划课题(2019YFC1712302,2019YFC1712304) （2019YFC1712302,2019YFC1712304）
江西省教育厅科技计划项目(GJJ201222) （GJJ201222）
江西省科技创新平台项目(20194AFD45001) （20194AFD45001）
中央引导地方科技发展专项项目(20192ZDD02002) （20192ZDD02002）
江西中医药大学校级创新创业训练计划项目(202110412213) （202110412213）
