中药材2023，Vol.46Issue(4)：925-933,9.DOI:10.13863/j.issn1001-4454.2023.04.022
UHPLC-MS/MS法同时测定三七中16个皂苷类成分在大鼠体内的含量及药代动力学研究
Simultaneous Determination of 16 Saponins Contents in Notoginseng Radix et Rhizoma in Rats by UHPLC-MS/MS and Pharmacokinetics
摘要
Abstract
Objective:To establish UHPLC-MS/MS method for simultaneous determination of 16 saponins in Notoginseng Radix et Rhizoma(ginsenosides Rf,Rg1,Rg2,Re,Rd,Rb1,Rb2,Rb3,Rc,Fa,Rk1,Rg5,Rg3,F2 and notoginseng saponins R1,Fc)in rat plasma,and to investigate their pharmacokinetic characteristics.Methods:The plasma samples were treated with methanol-acetonitrile(4:1)pre-cipitated protein,and ACQUITY UPLC BEH C18(100 mmx2.1 mm,1.7 μm)column was used.The mobile phase was 0.1％formic acid-acetonitrile with gradient elution;Using electrospray ion source(ESI),multiple response monitoring(MRM)was used to detect negative ions,DAS 3.0 software was used to calculate the pharmacokinetic parameters.Results:The 16 saponins had a good linear rela-tionship with the peak area in the corresponding concentration range(r＞0.9900).The RSD of intra-day and inter-day precision ranged from 0.88％to 14.41％,and accuracy ranged from 89.60％to 114.00％.The extraction recovery was 84.67％to 111.87％,and the matrix effect was 77.04％to 117.64％.Conclusion:The established UHPLC-MS/MS analytical method in the study is accurate and sen-sitive,and can be used for the content determination and pharmacokinetic study of the 16 saponins in Notoginseng Radix et Rhizoma in rats.关键词
三七/UHPLC-MS/MS/药代动力学/人参皂苷/三七皂苷Key words
Notoginseng Radix et Rhizoma/UHPLC-MS/MS/Pharmacokinetics/Ginsenoside/Notoginsenoside分类
医药卫生引用本文复制引用
欧阳慧子,吕振国,白宇,何俊..UHPLC-MS/MS法同时测定三七中16个皂苷类成分在大鼠体内的含量及药代动力学研究[J].中药材,2023,46(4):925-933,9.基金项目
国家自然科学基金项目(81673824) （81673824）
