中药材2023，Vol.46Issue(4)：975-979,5.DOI:10.13863/j.issn1001-4454.2023.04.031
天麻素改善线粒体损伤抑制LPS诱导的PC12细胞凋亡
Effect of Gastrodin on Mitochondrial Damage and Inhibition of LPS-Induced Apoptosis of PC12 Cells
摘要
Abstract
Objective:To investigate the protective effect of gastrodin on LPS induced apoptosis of PC12 cells using mitochondrial injury as entry point.Methods:The optimal intervention concentration of gastrodin was determined by MTT method,and normal control group,model group,Edaravone(0.005 mol/L)positive control group and gastrodin high(0.2 mol/L)and low(0.02 mol/L)concen-traiton groups were set up.The damage model of PC 12 cells was established by MTT method,and the damage condition was determined as 1 μg/mL LPS for 48 hours.ROS level was detected by DCFH-DA fluorescent probe and enzyme label instrument,mitochondrial membrane potential was detected by JC-1,mPTP openness and apoptosis were detected by flow cytometry,and the ratio of Bax/Bcl-2 in the cells was detected by Western Blot.Results:After LPS treatment,ROS levels in PC12 cells were significantly increased(P＜0.001),and the results of JC-1 and mPTP showed that the mitochondrial membrane potential significantly decreased(P＜0.01).After the inter-vention of gastrodin,ROS expression in PC 12 cells was significantly decreased and mitochondrial membrane potential was sibnificantly increased,the apoptosis rate and Bax/Bcl-2 level significantly decreased(P＜0.05 to P＜0.01).Conclusion:Gastrodin may inhibit apop-tosis by ameliorating mitochondrial damage in PC12 cells.关键词
神经退行性疾病/天麻素/线粒体损伤/氧化应激/细胞凋亡Key words
Neurodegenerative disease/Gastrodin/Mitochondrial damage/Oxidative stress/Apoptosis分类
医药卫生引用本文复制引用
向泉,潘际刚,陈婷婷,周雪,沈祥春..天麻素改善线粒体损伤抑制LPS诱导的PC12细胞凋亡[J].中药材,2023,46(4):975-979,5.基金项目
国家自然科学基金喀斯特中心项目(U1812403-4-4) （U1812403-4-4）
贵州省天然药物成药性人才基地(黔人颁发[2018]3号) （黔人颁发[2018]3号）
贵州省社会攻关计划项目(黔科合支撑[2016]2826) （黔科合支撑[2016]2826）
贵州特色天然药物资源高效利用工程中心(黔发改高技[2018]633号) （黔发改高技[2018]633号）
贵州省科技厅联合学术新苗项目(黔科合平台人才[2017]5718) （黔科合平台人才[2017]5718）
贵州医科大学药学国际科技合作基地(黔科合平台人才[2017]5802) （黔科合平台人才[2017]5802）
天然药物药理与成药性评价重点实验室(筑科合同[2017]5-21号) （筑科合同[2017]5-21号）
贵州省高层次创新型人才百层次人才项目(黔科合人才[2015]4029) （黔科合人才[2015]4029）
