中国现代中药2023，Vol.25Issue(10)：2072-2077,6.DOI:10.13313/j.issn.1673-4890.20230419001
UPLC指纹图谱结合化学模式识别评价不同产地五灵脂药材质量
Evaluation of Quality of Faeces Trogopterori from Different Producing Areas by UPLC Fingerprint and Chemical Pattern Recognition
摘要
Abstract
Objective:The ultra-high performance liquid chromatography(UPLC)fingerprint of Faeces Trogopterori was established,and the chemical pattern recognition method was used to evaluate the quality of Faeces Trogopterori from different producing areas.Methods:The fingerprint was established by UPLC,and the chromatographic column was ACQUITY UPLC HSS T3 column(150 mm×2.1 mm,1.8 μm).The mobile phase was acetonitrile-0.1%phosphoric acid solution(gradient elution);the detection wavelength was 265 nm;the flow rate was 0.25 mL·min-1;the column temperature was 30 ℃,and the injection volume was 1.0 μL.The similarity evaluation,cluster heat map analysis,and orthogonal partial least squares-discriminant analysis(OPLS-DA)were combined to evaluate the quality of Faeces Trogopterori from different producing areas.Results:A total of 14 common peaks were identified,and six components were identified in the established UPLC fingerprint of Faeces Trogopterori,which were protocatechuic acid,4-hydroxybenzoic acid,benzoic acid,quercitrin,amentoflavone,and hinokiflavone.The similarity of the 22 batches of Faeces Trogopterori was 0.870-0.998.Cluster heat map analysis was used to divide 22 batches of Faeces Trogopterori into two categories.The Faeces Trogopterori produced in Shangluo City of Shanxi Province could be completely distinguished from other producing areas.Seven markers of quality difference were selected by OPLS-DA.Conclusion:The UPLC fingerprint and chemical pattern recognition method of Faeces Trogopterori are stable and feasible,which can provide a reference for the quality study of Faeces Trogopterori.关键词
五灵脂/超高效液相色谱法指纹图谱/聚类热图分析/正交偏最小二乘法-判别分析Key words
Faeces Trogopterori/UPLC fingerprint/cluster heat map analysis/OPLS-DA分类
医药卫生引用本文复制引用
严玉晶,李玲,麦贵敏,曹嵌,林嘉明,邝敏,刘燎原..UPLC指纹图谱结合化学模式识别评价不同产地五灵脂药材质量[J].中国现代中药,2023,25(10):2072-2077,6.基金项目
工业和信息化部 2022 年产业技术基础公共服务平台项目(2022-230-221) （2022-230-221）
佛山市核心技术攻关项目(1920001000378) （1920001000378）
