中国现代中药2023，Vol.25Issue(10)：2084-2092,9.DOI:10.13313/j.issn.1673-4890.20230321005
藏族药甘青青兰不同花色花朵的代谢组学分析
Comparative Metabolomics Analysis on Different Flowers of Tibetan Medicine Dracocephalum tanguticum Maxim
摘要
Abstract
Objective:The non-targeted metabonomics of indigo and pink flowers of Tibetan medicine Dracocephalum tanguticum Maxim.were analyzed.It provides a reference for the quality evaluation and suitable application of D.tanguticum.Methods:Ultra-performance liquid chromatography-mass spectrometry(UPLC-MS)was used to analyze and identify the metabolites in different colors of flowers of D.tanguticum.Principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA)were used to analyze the differences of metabolites in different colors of flowers.The differential metabolites were annotated to Kyoto Encyclopedia of Genes and Genomes(KEGG),Human Metabolome Database(HMDB)and The Small Molecule Pathway Database(SMPDB),and the metabolic pathways and enrichment of the differential metabolites were characterized by MetaboAnalyst.Results:A total of 560 metabolites are identified from two different flower color samples.There are 223 lipids and lipids,77 oxygen-containing organic compounds,76 phenylpropanoids and polyketones,74 organic heterocyclic compounds,59 organic acids and their derivatives,31 benzene ring derivatives,10 nitrogen-containing organic compounds,four nucleosides/nucleotides and analogues,four lignans/neolignans and related compounds,one hydrocarbon derivative,and one alkaloid and its derivatives.There are 33 significant differences between indigo flowers and pink flowers.Compared with indigo flowers,20 metabolites in pink flowers are up-regulated,and 13 metabolites are down-regulated.Conclusion:In this paper,the secondary metabolites of different flower colors of D.tanguticum are clarified,and the differential metabolites and metabolic pathways of indigo flowers and pink flowers are clarified,which can provide basic data for the development and application of Tibetan medicine D.tanguticum.关键词
甘青青兰/紫蓝色花/粉色花/次生代谢产物/非靶向代谢组学Key words
Dracocephalum tanguticum Maxim./indigo flower/pink flower/secondary metabolites/non-targeted metabolomics分类
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赵翔,赛曼,张艳鹏,于晓锐,王勇,兰世超,申振,段亚玲..藏族药甘青青兰不同花色花朵的代谢组学分析[J].中国现代中药,2023,25(10):2084-2092,9.基金项目
中央本级重大增减支项目(2060302) （2060302）
第四次全国中药资源普查[尼玛县中(藏)药资源普查项目](20191212-542430) （藏）
