中国现代中药2023，Vol.25Issue(10)：2103-2108,6.DOI:10.13313/j.issn.1673-4890.20230412001
水苏糖抗衰老活性及机制研究
Study on Anti-aging Activity and Mechanism of Stachyose
摘要
Abstract
The anti-aging activity and mechanism of action of stachyose in vivo were investigated using the Cryptobacterium hidradenum nematode model(hereafter referred to as nematode).Methods:The antioxidant effect of stachyose was studied by measuring the body length,head swing frequency,lipofuscin,oxidative stress and thermal stress of wild-type N2 nematodes.The molecular mechanism of stachyose anti-aging was discussed by measuring the nuclear translocation of decay accelerating factor-16(DAF-16)protein of transgenic TJ356 nematode and the expression of superoxide dismutase-3(SOD-3)in CF1553 nematode.Results:It was found that stachyose not only significantly improved the head swing frequency of nematodes(P<0.001)but also significantly reduced lipofuscin deposition(P<0.001)and significantly prolonged the heat tolerance of nematodes(P<0.01).In addition,stachyose can promote the translocation of DAF-16 nucleus of TJ356 nematode and significantly increase the expression level of SOD-3 protein in CF1553 nematode.Conclusion:The antioxidant effect of stachyose was significant,and this effect could be attributed to insulin/insulin-like growth factor 1 signal pathway(IIS)signaling,which promoted DAF-16 transcription and thus stimulated SOD-3 expression,resulting in cell protection.关键词
水苏糖/秀丽隐杆线虫/抗衰老活性/抗氧化活性Key words
stachyose/Cryptobacterium hidradenum nematode/anti-aging activity/antioxidant activity分类
医药卫生引用本文复制引用
陈旭,梁佳政,李志恒,丁克林,高雅婷,柳志诚,刘永刚,张园园..水苏糖抗衰老活性及机制研究[J].中国现代中药,2023,25(10):2103-2108,6.基金项目
国家重点研发计划项目(2018YFC1706505) （2018YFC1706505）
