中医药信息2023，Vol.40Issue(12)：23-28,34,7.DOI:10.19656/j.cnki.1002-2406.20231205
美洲大蠊提取物体外对肝癌耐药细胞BEL-7402/5-FU自噬和侵袭转移的影响
Effects of Extracts from Periplaneta americana L.on Autophagy,Invasion and Metastasis of Hepatocarcinoma Drug-Resistant Cells BEL-7402/5-FU in vitro
摘要
Abstract
Objective:To investigate the effects of Periplaneta americana L.extract CⅡ-3 and the skimmed cream on the autophagy and metastatic invasion ability of 5-FU resistant hepatocellular carcinoma cells(Bel-7402/5-FU).Methods:Hepatocellular carcinoma-sensitive cells BEL-7402 and BEL-7402/5-FU were treated with sorafenib,CⅡ-3 and the skimmed cream to detect the production of autophagic marker proteins,as well as the levels of autophagy-related factors and invasion and metastasis-related factors in the cells and the changes in the invasion and metastasis ability of the cells.Results:P62 protein expression was low in the drug-resistant cell group,and the expression significantly increased after administration of positive drugs sorafenib,CⅡ-3 and the skimmed cream.Compared with sensitive cells,LC3B production increased in drug-resistant cells and decreased after administration of CⅡ-3.Both CⅡ-3 and the skimmed cream administration groups significantly inhibited the expression of some factors related to autophagy and invasive metastasis,including mRNA expression of ATG5,PIK3C3,BECLIN,LC3B,MMP-9,AKT,and 4EBP1.The results of scratch test showed that drug-resistant cells had stronger invasive metastasis ability compared with sensitive cells,and the administration of either sorafenib or CⅡ-3 significantly inhibited the invasive metastasis of the cells,while no significant inhibitory effect was observed for desiccated cream.Conclusion:Drug-resistant cells of hepatocellular carcinoma have strong autophagy and invasive metastasis ability,while both CⅡ-3 and skimmed cream of Periplaneta americana L.can significantly inhibit the autophagy and invasive metastasis of drug-resistant cells in vitro,but can only affect some related factors.关键词
美洲大蠊提取物/肝癌/自噬/侵袭转移Key words
Periplaneta americana L.extract/Hepatocellular carcinoma/Autophagy/Invasion and metastasis引用本文复制引用
马璟婷,周杰,李彩琳,吴定宇,彭芳..美洲大蠊提取物体外对肝癌耐药细胞BEL-7402/5-FU自噬和侵袭转移的影响[J].中医药信息,2023,40(12):23-28,34,7.基金项目
国家自然科学基金项目(81560600) （81560600）
