组织工程与重建外科杂志2023，Vol.19Issue(6)：525-529,540,6.DOI:10.3969/j.issn.1673-0364.2023.06.001
miR-148a-3p调控血管生成的体内外表型鉴定
In vivo and vitro phenotype identification of miR-148a-3p regulating angiogenesis
摘要
Abstract
Objective To identify the phenotype of miR-148a-3p in regulating angiogenesis.Methods The primary tissues of miR-148a knock-out mice were extracted and immunohistochemical staining was used to investigate the effect of miR-148a knockout on angiogenesis.By means of miRNA Mimic transfection,miR-148a-3p was overexpressed in human umbilical vein endothelial cells(hUVECs).Thereafter,the influence of miR-148a-3p overexpression on angiogenesis was studied in aspects of Matrigel-based tube forming assay,cell proliferation,cell migration,qPCR,etc.The effects of miR-148a-3p on angiogenesis were clarified in terms of knock-out and overexpression.Results Compared with heterozygous littermates,the miR-148a knock-out mice had an abnormal distribution of type-H vessels in the bone tissue.The ex-vivo fetal metatarsal experiments further confirmed the enhanced angiogenesis ability in absence of miR-148a.The expression level of miR-148a-3p was overexpressed by miR-148a-3p mimic in vascular endothelial cells.It was found that the overexpression of miR-148a-3p did not affect the proliferation ability of hUVECs,but could interfere with angiogenesis by inhibiting its migration ability,and affect the formation of lamellipodia and tubes.Conclusion miR-148a-3p can inhibit angiogenesis both in-vivo and in-vitro,including sprouting,migration,and tube formation.关键词
miR-148a-3p/血管生成/基因敲除小鼠/表型鉴定/H型血管Key words
miR-148a-3p/Angiogenesis/Gene knock-out mice/Phenotype identification/Type H vessels分类
生物科学引用本文复制引用
黄瑾,韩文卿,李鑫,陈骁俊..miR-148a-3p调控血管生成的体内外表型鉴定[J].组织工程与重建外科杂志,2023,19(6):525-529,540,6.基金项目
上海市科学技术委员会启明星培育(扬帆专项,23YF1421700) （扬帆专项,23YF1421700）
上海市科学技术委员会临床医学研究中心项目(22MC1940300). （22MC1940300）
