长春中医药大学学报2023，Vol.39Issue(12)：1334-1338,5.DOI:10.13463/j.cnki.cczyy.2023.12.008
蒙医温针下调脊髓背角小胶质细胞STAT3缓解大鼠脊髓损伤后坐骨神经痛
Down-regulation of STAT3 of spinal dorsal horn microglia through Mongolian medical warm acupuncture to relieve sciatica after spinal cord injury in rats
摘要
Abstract
Objective To explore the significance of Mongolian medical warm acupuncture in relieving sciatica after spinal cord injury in rats.Methods SPF healthy male Wistar rats aged 3 months were selected and randomly divided into five groups,namely the normal control group(normal group,n=15),sham operation group(sham group,n=20),spinal cord blunt injury model group(CNP group,n=30),STAT3 siRNA injection group(n=30)and Mongolian medical warm acupuncture treatment group(n=30).In Mongolian medical warm acupuncture treatment group,the knee-eye points(the depressions on both sides of the patellar ligament)and the hip points(the midpoint of the gluteal transverse stripes)were taken and treated with special silver needles.The results of behavioral scores of rats in each group were compared,and the behavioral effects of rats after Mongolian medical warm acupuncture treatment and intrathecal injection of STAT3 siRNA were counted.Results The BBB score of rats in CNP group on 28th day after modeling was higher than that on 1st,7th,14th and 21st day after modeling(P＜0.05),and paw withdrawal thermal duration in CNP group from the 3rd day after modeling was shorter than that in sham group and normal group(P=0.032).On the 3rd day after operation,mechanical induction foot threshold in CNP group was lower than that in sham group and normal group(P=0.021).Compared with normal group,sham group and the airborne bacteria,in Mongolian medical warm acupuncture treatment group and STAT3 siRNA injection group the TWD time was significantly decreased(P＜0.01),while MWT was significantly increased(P＜0.01)and BBB score was significantly increased(P＜0.01),and after Mongolian medical warm acupuncture treatment the expression of STAT3 in spinal cord was significantly decreased on the 7th,14th and 28th day after the modeling(P＜0.01).The expression of STAT3 mRNA in the Mongolian medical warm acupuncture treatment group and STAT3 siRNA injection group was significantly lower than that in normal group,sham group and the airborne bacteria.Conclusion The activation of STAT3 signal transduction pathway in microglia may be one of the pathogenesis of central sciatica after spinal cord injury,and Mongolian medical warm acupuncture treatment may play a clinical role by blocking the activity of STAT3 signal transduction pathway in microglia in spinal dorsal horn.关键词
脊髓钝性损伤/中枢神经痛/STAT3/蒙医温针/STAT3 siRNA/发病机制Key words
blunt spinal cord injury/central neuralgia/STAT3/Mongolian medical warm acupuncture/STAT3 siRNA/pathogenesis分类
医药卫生引用本文复制引用
斯楞格,阿古拉,阿日嘎太,包立道,阿茹娜..蒙医温针下调脊髓背角小胶质细胞STAT3缓解大鼠脊髓损伤后坐骨神经痛[J].长春中医药大学学报,2023,39(12):1334-1338,5.基金项目
国家自然科学基金项目(82074577,82160945) （82074577,82160945）
内蒙古自然科学基金项目(2020MS08125) （2020MS08125）
内蒙古医科大学蒙医学"一流学科"建设项目(myxylxk2022023,myxylxk2022025) （myxylxk2022023,myxylxk2022025）
内蒙古医科大学蒙医学"一流学科"青年教师科研创新项目(MYX2023-S01) （MYX2023-S01）
内蒙古自治区蒙医药协同创新中心科研项目(MYYXTPY202207) （MYYXTPY202207）
内蒙古医科大学面上项目(YKD2021MS049) （YKD2021MS049）
内蒙古自治区卫健委医疗卫生科技计划项目(202201212) （202201212）
