福建农林大学学报（自然科学版）2024，Vol.53Issue(1)：69-78,10.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202302013
柯乐猪异常乳腺组织的TMT蛋白组学分析
Proteomic analysis of abnormal mammary gland tissue of Kele pig based on TMT technique
摘要
Abstract
[Objective]Explore the regulatory mechanisms of mammary gland development in Kele pigs using tandem mass tag(TMT)quantitative proteomics technology,providing a basis for breeding efforts.[Method]Three normal and three abnormal mam-mary gland tissues were collected from Kele sow pigs.TMT quantitative proteomics technology was employed to investigate the protein expression levels in normal and abnormal mammary gland tissues.Differential key proteins related to mammary gland development were screened,and bioinformatics analysis was conducted on the differentially expressed proteins.[Result]The results revealed 474 differentially expressed proteins,including 245 upregulated and 229 downregulated proteins.Gene ontology(GO)functional enrich-ment analysis indicated that these differentially expressed proteins were mainly enriched in biological processes such as epithelial cell differentiation,skin development,and renal tubule epithelial cell process.They were predominantly localized in extracellular regions and extracellular spaces,and involved in functions like peptidase inhibitor activity and peptidyl-cysteine activity.Kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis revealed that these differentially expressed proteins were mainly enriched in sig-naling pathways related to complement system,peroxisome proliferator activated receptor,and Staphylococcus aureus infection.Dif-ferential protein interaction network analysis identified 10 key differentially expressed proteins,which included upregulated proteins of P14287(osteopontin),F1RXF9(keratin 25),I3LDS3(keratin 10),Q75QW1(epithelial cell adhesion molecule),A0A5S8KLN1(cornulin)and F1RW75(grifin),and downregulated proteins of A0A286ZT13(albumin),P06867(plasmino-gen),F1SCC9(protein containing serine protease inhibitor domain)and P50447(α-1-antitrypsin).[Conclusion]TMT quantitative proteomics was proved to be an effective technique in screening differentially expressed proteins between normal and abnormal mam-mary gland tissues of sows.关键词
柯乐猪/串联体量标记(TMT)技术/乳腺组织/差异表达蛋白Key words
Kele pig/tandem mass tags(TMT)technology/mammary gland tissue/differentially expressed protein分类
农业科技引用本文复制引用
王春源,熊力,杨红文,郭小江,张依裕,谭元成,杨酸..柯乐猪异常乳腺组织的TMT蛋白组学分析[J].福建农林大学学报（自然科学版）,2024,53(1):69-78,10.基金项目
贵州省科技支撑计划项目(黔科合支撑[2021]147 号) （黔科合支撑[2021]147 号）
贵州省优秀青年科技人才培养计划项目(黔科合平台人才[2021]5630号) （黔科合平台人才[2021]5630号）
贵州省生猪产业发展项目(2021). （2021）
