福建农林大学学报（自然科学版）2024，Vol.53Issue(1)：89-95,7.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202301020
东方蜜蜂微孢子虫侵染过程中nce-miR-15338及候选靶基因的表达谱
Expression patterns of nce-miR-15338 and candidate target genes during the infection process of Nosema ceranae
摘要
Abstract
[Objective]This study aims to provide reference and basis for exploring the function and action mechanism underlying the nce-miR-15338 regulated Nosema ceranae infection of Apis mellifera ligustica workers.[Method]Stem-loop RT-PCR and Sanger se-quencing were firstly applied to verify the existence and expression of nce-miR-15338.After the prediction of its target genes and da-tabase annotation by related software,the expression patterns of nce-miR-15338 and its candidate target genes during N.ceranae′s in-fection of A.mellifera ligustica workers were determined by RT-qPCR.[Result]The results proved the existence and expression of nce-miR-15338 in N.ceranae spores.Nce-miR-15338 potentially targeted 16 genes,including LCFAL 2,20s PS,etc.,and up to 16,7,8 and 3 target genes were annotated to Nr,Swiss-Prot,GO and KEGG databases,respectively.Compared with its expression level 1 day post inoculation(1 d),the expression level of nce-miR-15338 was significantly up-regulated at 2 d,while significantly down-regulated at 4,6 and 8 d;the expression levels of LCFAL 2 and 20sPS were both significantly down-regulated at 2,4,6 and 8 d.[Conclusion]The study verified the authentic existence of nce-miR-15338 in N.ceranae,and clarified the expression patterns of nce-miR-15338 as well as its candidate target genes of LCFAL 2 and 20sPS during N.ceranae′s infection in A.m.ligustica workers.关键词
东方蜜蜂微孢子虫/蜜蜂/意大利蜜蜂/nce-miR-15338/靶基因/表达谱Key words
Nosema ceranae/honey bee/Apis mellifera ligustica/nce-miR-15338/target genes/expression pattern分类
农业科技引用本文复制引用
王思懿,郭睿,高旭泽,赵浩东,荆欣,刘小玉,冯佩林,宋宇轩,刘彩珍,陈大福..东方蜜蜂微孢子虫侵染过程中nce-miR-15338及候选靶基因的表达谱[J].福建农林大学学报（自然科学版）,2024,53(1):89-95,7.基金项目
国家自然科学基金项目(32172792) （32172792）
国家现代农业产业技术体系建设专项资金项目(CARS-44-KXJ7) （CARS-44-KXJ7）
福建省自然科学基金项目(2022J01131334) （2022J01131334）
福建农林大学硕士生导师团队项目 （）
福建农林大学动物科学学院(蜂学学院)科研扶持项目 （蜂学学院）
福建省大学生创新创业训练计划项目(202210389121,202210389126). （202210389121,202210389126）
