福建农林大学学报（自然科学版）2024，Vol.53Issue(1)：114-122,9.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202302030
水栒子内生真菌群落结构及多样性
Community structure and diversity of endophytic fungi in Cotoneaster multiflorum
摘要
Abstract
[Objective]The study aimed to explore the diversity of endophytic fungi and community structure in different organs of C.multiflorus,and provided a theoretical basis for the comprehensive development and utilization of endophytic fungi in C.multiflorus.[Method]High-throughput sequencing technology was used to identify and analyze the endophytic fungi in different tissues of C.mul-tiflorus.[Result]A total of 1 590 OTU sequences were obtained from high-throughput sequencing,including 597 OTUs detected in the stems,545 OTUs in the leaves and 448 OTUs in the roots.Diversity analysis showed certain differences in the community struc-ture and abundance of endophytic fungi between different tissues of C.multiflorus.The dominant phylum in all tissues was Ascomyco-ta,with relative abundances of 41.65%in the roots,22.29%in the stems,and 10.16%in the leaves.At the genus level,the domi-nant genera(relative abundance>2.00%)were Spirosphaera(8.89%),Anguillospora(7.92%)and Subulicystidium(2.10%)in the roots,Didymella(2.52%)and Arthrocatena(2.19%)in the stems,and Filobasidium(2.47%)and Pyrenochaeta(2.05%)in the leaves.LEfSe analysis further revealed the indicator species for different tissues,which were Spirosphaera for root,Arthrocatena for stem,and Filobasidium for leaf.[Conclusion]The study revealed the differences on fungal community structure in various tis-sues of C.multiflorus,and abundant endophytic fungal resources in the tissue organs of C.multiflorus were found.关键词
水栒子/内生真菌/高通量测序/多样性/群落组成Key words
Cotoneaster multiflorus/endophytic fungi/high-throughput sequencing/diversity/community composition分类
农业科技引用本文复制引用
吕增伟,文国卫,赖文峰,施晨阳,陈启华,刘益鹏,黄秋良,张国防..水栒子内生真菌群落结构及多样性[J].福建农林大学学报（自然科学版）,2024,53(1):114-122,9.基金项目
福建省科技计划项目(2022I0008) （2022I0008）
东西部协作项目(KH180062A、11891008004、KH190315A) （KH180062A、11891008004、KH190315A）
福建农林大学科技创新基金(CXZX2019046). （CXZX2019046）
