福建农林大学学报（自然科学版）2024，Vol.53Issue(1)：129-135,7.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202212036
栀子花多糖的提取工艺及其脂代谢调节作用
Research on extraction and lipid regulatory effect of the polysaccharides from Gardenia jasminoides flower
摘要
Abstract
[Objective]To develop polysaccharide resources from Gardenia Jasminoides Ellis flowers,we studied the extraction process of G.Jasminoides flower polysaccharides and their lipid metabolism regulation effects.[Method]Single factor experiment and response surface methodology were used to optimize the extraction process of G.jasminoides flower polysaccharide in this study.Oleic acid was used to establish fat accumulation HepG2 cell model,to evaluate the effect of the polysaccharide on the lipid metabolism in HepG2 fat accumulation cells.[Result]The extraction rate of G.jasminoides polysaccharides was maximized at 17.48%with liquid-material ratio of 25 mL·g-1,ultrasonic power of 550 W,ultrasonic time of 140 min,and extraction temperature of 63℃.Com-pared with the model group,G.jasminoides flower polysaccharides could effectively reduce the contents of triglyceride(TG),total cholesterol(TC)and low-density lipoprotein cholesterol(LDL-C)while increase the content of high-density lipoprotein cholesterol(HDL-C)in high-fat cells,without impairing the survival rate of HepG2 cells.[Conclusion]The anti-hyperlipidemic activity of G.jasminoides flower polysaccharide was good.关键词
栀子花/多糖/提取工艺/脂代谢调节Key words
Gardenia jasminoides flower/polysaccharides/extraction/lipid metabolism regulation分类
农业科技引用本文复制引用
柳宛艺,邓湘铸,尚煜昆,黄超,吴思蓥,黄维..栀子花多糖的提取工艺及其脂代谢调节作用[J].福建农林大学学报（自然科学版）,2024,53(1):129-135,7.基金项目
福建省高校产学合作项目(2022N5009) （2022N5009）
福建省林业科技项目(闽林科[2020]29 号) （闽林科[2020]29 号）
福建省林业局科技研究项目(闽林函[2020]25 号). （闽林函[2020]25 号）
