嗜肺军团菌效应蛋白Lpg1972与CNOT7相互作用研究OACSTPCD

Legionella pneumophila is a Gram-negative pathogenic bacterium that parasitizes within eukaryotic cells.Its pathogenicity primarily relies on the secretion of over 330 effector pro-teins through the Type IVB secretion system.These effector proteins collaborate to collectively inter-fere with various cellular activities of the eukaryotic host cell.The Legionella pneumophila effector protein Lpg1972 has been identified to interact with the CNOT7 subunit of the Ccr4-Not complex in eukaryotic cells.The Ccr4-Not complex predominantly regulates mRNA degradation in eukaryotic cells.To validate this interaction,molecular biology techniques were employed,involving the de-sign of a co-expression vector to purify the protein complex.The complex protein was successfully obtained and its structure and interaction were predicted using AlphaFold2.These analyses lay a foundational basis for subsequent research,establishing a comprehensive understanding of the struc-tural and functional aspects of the identified interaction.