福建师范大学学报（自然科学版）2024，Vol.40Issue(1)：23-33,11.DOI:10.12046/j.issn.1000-5277.2024.01.003
嗜肺军团菌效应蛋白Lpg1972与CNOT7相互作用研究
Interaction of Legionella pneumophila Effector Protein Lpg1972 with CNOT7
摘要
Abstract
Legionella pneumophila is a Gram-negative pathogenic bacterium that parasitizes within eukaryotic cells.Its pathogenicity primarily relies on the secretion of over 330 effector pro-teins through the Type IVB secretion system.These effector proteins collaborate to collectively inter-fere with various cellular activities of the eukaryotic host cell.The Legionella pneumophila effector protein Lpg1972 has been identified to interact with the CNOT7 subunit of the Ccr4-Not complex in eukaryotic cells.The Ccr4-Not complex predominantly regulates mRNA degradation in eukaryotic cells.To validate this interaction,molecular biology techniques were employed,involving the de-sign of a co-expression vector to purify the protein complex.The complex protein was successfully obtained and its structure and interaction were predicted using AlphaFold2.These analyses lay a foundational basis for subsequent research,establishing a comprehensive understanding of the struc-tural and functional aspects of the identified interaction.关键词
嗜肺军团菌/大肠杆菌/Ccr4-Not复合物/相互作用Key words
Legionella pneumophila/Escherichia coli/Ccr4-Not complex/interaction分类
资源环境引用本文复制引用
刘子赫,吴书娴,甄向凯..嗜肺军团菌效应蛋白Lpg1972与CNOT7相互作用研究[J].福建师范大学学报（自然科学版）,2024,40(1):23-33,11.基金项目
国家自然科学基金面上项目(32170045) （32170045）
