基于RPA的病原体快速诊断策略OACSTPCD

Recombinase polymerase amplification(RPA)is an isothermal nucleic acid ampli-fication technique that emerged in 2006.The primer design for RPA is simple,and the reaction time is short.At temperatures ranging from 37 to 42℃,it takes only 15～20 min to reach the pathogen detection level.When coupled with convenient devices,it can build a nucleic acid detec-tion platform.In recent years,RPA has undergone rapid development.This article focuses on the strategies for detecting RPA reaction products and provides a review of the rapid diagnostic applica-tion of RPA in pathogenic bacteria,viruses,and parasites.