福建师范大学学报（自然科学版）2024，Vol.40Issue(1)：34-44,11.DOI:10.12046/j.issn.1000-5277.2024.01.004
基于RPA的病原体快速诊断策略
RPA-based Rapid Diagnostic Strategies for Pathogens
摘要
Abstract
Recombinase polymerase amplification(RPA)is an isothermal nucleic acid ampli-fication technique that emerged in 2006.The primer design for RPA is simple,and the reaction time is short.At temperatures ranging from 37 to 42℃,it takes only 15～20 min to reach the pathogen detection level.When coupled with convenient devices,it can build a nucleic acid detec-tion platform.In recent years,RPA has undergone rapid development.This article focuses on the strategies for detecting RPA reaction products and provides a review of the rapid diagnostic applica-tion of RPA in pathogenic bacteria,viruses,and parasites.关键词
等温扩增/重组酶聚合酶扩增/侧向流动/实时荧光检测/病原体检测Key words
isothermal amplification/recombinase polymerase amplification/lateral flow/real-time fluorescence detection/pathogen detection分类
医药卫生引用本文复制引用
许淑莹,王冬梅,欧阳松应..基于RPA的病原体快速诊断策略[J].福建师范大学学报（自然科学版）,2024,40(1):34-44,11.基金项目
国家自然科学基金资助项目(82172287) （82172287）
