不同更新方式下亚热带森林土壤病毒群落结构与功能特征OACSTPCD

This study aimed to explore the characteristics of soil viral communities and their roles in biogeochemical cycling under different forest restoration patterns.Viral metagenomics and bioinformatics methods were employed to examine the composition and functionality of viral commu-nities in a Chinese fir plantation,a natural regeneration forest,and a primeval forest.The results showed that the mode of forest regeneration had a significant impact on the structure of the soil viral community.Siphoviridae demonstrated prevalence in the naturally regenerating and primeval forests(62.60%and 31.49%,respectively),while the Microviridae were observed to be the most abundant in the fir plantation(27.89%).The prevalence of nucleo-cytoplasmic large DNA viruses in the examined soils was highest in the natural regeneration forest(20.83%).Proteobacteria were the dominant viral host in the fir plantation and natural regeneration forest,whereas Actinobacteria were prevalent in the primeval forest.Soil viral hosts in the three forest types commonly include pathogenic bacteria that can infect humans and animals,such as Gordonia,Rhodococcus,and Mycolicibacterium.Rich auxiliary carbohydrate-active enzyme(CAZyme)genes were detected in the functional groups of viromes.The number of genes encoding CAZymes in soil viruses of fir planta-tions(4)was significantly lower compared to primary forests(210)and natural regeneration forests(69).These findings reveal the potential impact of forest renewal patterns on soil viral structure,hosts,and carbon cycle function.