福建师范大学学报（自然科学版）2024，Vol.40Issue(1)：69-75,7.DOI:10.12046/j.issn.1000-5277.2024.01.008
长期施肥处理对东北黑土真菌多样性和群落结构的影响
Effect of Long-term Fertilization on Black Soil Fungal Community Diversity and Structure in Northeast China
摘要
Abstract
Based on a 40-year long-term nitrogen and phosphorus localization experiment,we utilized high-throughput sequencing technique to comparatively analyze the differences in black soil fungal communities under four treatments:no fertilization(CK),nitrogen fertilizer(N),phos-phorus fertilizer(P),and a combination of nitrogen and phosphorus(NP).The results showed that,compared with CK,long-term nitrogen input significantly reduced soil pH,and significantly increased soil NO-3-N,NH+4-N,and soluble organic nitrogen(DON)contents,while phosphorus input significantly increased soil available phosphorus and total phosphorus contents.The most abun-dant phyla of soil fungi were Ascomycota(relative abundance 63%～68%),Mucoromycota(rela-tive abundance 15%～20%),and Basidiomycota(relative abundance 9%～15%).Different fertili-zation treatments had a significant impact on fungal diversity.In the NP treatment,the number of soil fungal amplicon sequence variants(ASVs)and Shannon diversity were 405 and 4.16,which were significantly lower than those in the CK treatment,around 502 and 4.64,respectively.Non-metric multidimensional scaling analysis revealed that the fungal community structures in NP and N treatments were significantly separated from CK and P treatments.Redundancy analysis showed that soil properties,including pH,NO-3-N,and DON were the main influencing factors on soil fungal community structure.These findings suggest that long-term nitrogen and phosphorus fertilization treatments have a significant impact on soil fungal communities via affecting soil properties.关键词
氮磷输入/真菌群落/土壤酸化/长期施肥/黑土Key words
nitrogen and phosphorus input/fungal community/soil acidification/long-term fertilization/black soil分类
农业科技引用本文复制引用
邓慧玉,刘子恺,马星竹,郝小雨,赵月,沈菊培,贺纪正..长期施肥处理对东北黑土真菌多样性和群落结构的影响[J].福建师范大学学报（自然科学版）,2024,40(1):69-75,7.基金项目
国家自然科学基金资助项目(32171642) （32171642）
福建省"闽江学者奖励计划"项目(2021) （2021）
