口腔疾病防治2024，Vol.32Issue(2)：89-100,12.DOI:10.12016/j.issn.2096-1456.2024.02.002
基于网络药理学探讨新疆桑白皮防龋机制及其对主要致龋菌作用的研究
Caries prevention mechanism of the Xinjiang Mori Cortex based on network pharmacology and its effect on the main cariogenic bacteria
摘要
Abstract
Objective To investigate the potential caries prevention mechanism of the Xinjiang Mori cortex and to analyze its effect on the main cariogenic bacteria.Methods The active components of the Xinjiang Mori cortex and the main targets were predicted and screened using the TCMSP database.The GeneCards,DisGENET and TTD databas-es were used to obtain caries-related targets.The common targets were derived,and core genes were screened.The en-richment analysis was performed using the DAVID data platform.Molecular docking was performed using AutoDock software.In in vitro antibacterial experiments,first,the 50％minimum inhibitory concentration(MIC50)and the mini-mum bactericidal concentration(MBC)of the Xinjiang Mori Cortex extract against Streptococcus mutans,Streptococcus sanguis and Actinomyces viscosus were determined and the growth curves were measured.The effects of the Xinjiang Mo-ri Cortex extract on acid production,polysaccharide production and adhesion ability of Streptococcus mutans,Streptococ-cus sanguis and Actinomyces viscosus in the planktonic state were determined.The 50％minimum biofilm inhibition con-centration(MBIC 50)and 50％minimum biofilm reduction concentration(MBRC50)were determined by crystal violet stain-ing,and biofilm morphology was visualized using scanning electron microscopy(SEM).Results The main active com-ponents of the Xinjiang Mori cortex included quercetin,kaempferol,and β-sitosterol.Tumor necrosis factor(TNF),inter-leukin-6(IL-6),and interleukin-1 beta(IL-1β)could be the most important targets of the Xinjiang Mori cortex for the prevention of dental caries.The enrichment analysis results showed that Mori cortex extract may have effects on the AGE-RAGE signaling pathway,IL-17 signaling pathway,and TNF signaling pathway.The antibacterial experiment re-sults showed that the MIC50 values of Xinjiang Mori Cortex extract against Streptococcus mutans,Streptococcus sanguis and Actinomyces viscosus were 0.5,0.5 and 0.25 mg/mL,respectively,and the MBCs were 4.0,2.0 and 1.0 mg/mL,re-spectively.The inhibitory effect of Xinjiang Mori Cortex extract on the acid production,polysaccharide production and adhesion ability of three major cariogenic bacteria in the planktonic state was stronger than that of the control group,and the differences were statistically significant(P＜0.05).The MBIC50 was 1.0,1.0,and 0.5 mg/mL,and the MBRC50 was 4.0,4.0,and 2.0 mg/mL.SEM observation showed that the amount of biofilm formation decreased with the drug concen-tration compared with the control group.Conclusion Xinjiang Mori cortex extract can prevent caries through querce-tin,kaempferol,and β-sitosterol active ingredients,TNF、IL-6、IL-1 β key targets and multiple pathways and inhibit the growth,acid production,polysaccharide production,and adhesion ability of three major cariogenic bacteria in the plank-tonic state and has some inhibitory effect on corticogenic biofilm formation.关键词
网络药理学/分子对接/天然药物/桑白皮/提取物/龋病/防龋/致龋细菌/抑菌/毒力因子Key words
network pharmacology/molecular docking/natural medicine/Mori Cortex/extract/caries/caries prevention/cariogenic bacteria/bacteriostasis/virulence factor分类
医药卫生引用本文复制引用
阿尔曼·阿卜力孜,吴龙,玛依奴尔·阿塔吾拉,赵今..基于网络药理学探讨新疆桑白皮防龋机制及其对主要致龋菌作用的研究[J].口腔疾病防治,2024,32(2):89-100,12.基金项目
新疆维吾尔自治区自然科学基金(2022D01C751) （2022D01C751）
新疆医科大学第一附属医院"青年科研起航"专项基金(2022YFY-QKQN-40)This study was supported by the grants from Natural Science Foundation of Xinjiang Uygur Autonomous Region(No.2022D01C751)and"Youth Research Launch"Special Foundation of the First Affiliated Hospital of Xinjiang Medical University(No.2022YFY-QKQN-40). （2022YFY-QKQN-40）
