广东医学2023，Vol.44Issue(10)：1223-1229,7.DOI:10.13820/j.cnki.gdyx.20230398
NETO2介导的ERK通路表达与口腔鳞状细胞癌转移和预后的关系
Correlations between the expression of ERK pathway mediated by NETO2 and metastasis and prognosis of oral squamous cell carcinoma
摘要
Abstract
Objective To investigate the clinical significance of neuropilin and tolloid-like 2(NETO2)in oral squamous cell carcinoma(OSCC)and the effect of its knockdown on OSCC cell proliferation,migration and invasion.Methods NETO2 expression in OSCC and non-OSCC tissues of OSCC patients was analyzed using Gene Expression Profile Interaction Analysis(GEPIA),and the correlation of NETO2 expression with overall survival in OSCC patients was analyzed.From November 2020 to January 2022,124 cancer specimens and matched normal tissues(5cm from the tumor edge)were obtained from our hospital.NETO2 expression was analyzed by Western blotting.In in vitro experiments,HSC-4 cells were divided into NETO2 knockdown(sh1,sh2)group and corresponding control(NC)group.The effects of NETO2 on HSC-4 cell proliferation,invasion and migration were investigated using CCK-8,clone formation and Tr-answell assays.In addition,rescue experiments were performed by adding an ERK agonist(EGF)to NETO2-knockdown HSC-4 cells.Results The expression of NETO2 in OSCC tissues was abnormally high,and the prognosis of OSCC pa-tients with high NETO2 expression was poor.Compared with the NC group,the cell viability,colony-forming ability,and the number of migrating and invasive cells in the sh1 and sh2 groups were significantly decreased(P＜0.05).Gene Set Enrichment Analysis(GSEA)of Gene Ontology(GO)revealed that knockdown of NETO2 in OSCC cells resulted in significant changes in ERK signaling pathway gene expression.Compared with sh1 group,sh1+EGF group increased the expression of ERK in NETO2 knockdown cells(P＜0.05).In addition,the growth,colony formation ability,migration and invasion of NETO2 knockdown OSCC cells in the sh1+EGF group were significantly higher than those in the sh1 group(P＜0.05).Conclusion High levels of NETO2 expression in OSCC tissues are associated with poor patient out-comes.NETO2 is an important oncoprotein that may activate the ERK signaling pathway to promote the proliferation,inva-sion and metastasis of OSCC cells.关键词
神经纤毛及唾样蛋白2/口腔鳞状细胞癌/ERK通路/增殖/转移Key words
neuropilin and tolloid-like 2/oral squamous cell carcinoma/ERK pathway/proliferation/metastasis分类
医药卫生引用本文复制引用
陆珣珣,韦曙平,韦富贵..NETO2介导的ERK通路表达与口腔鳞状细胞癌转移和预后的关系[J].广东医学,2023,44(10):1223-1229,7.基金项目
广西壮族自治区卫生健康委员会自筹经费科研课题(Z-B20220958) （Z-B20220958）
