MiR-184通过调节FOXO3促进卵巢癌细胞增殖能力OACSTPCD
MiR-184 promotes the proliferation ability of ovarian cancer cells by regulating FOXO3
目的 探讨miR-184通过调节FOXO3促进卵巢癌细胞增殖能力的影响.方法 荧光定量PCR检测样品中miR-184的表达水平;将miR-184mimic和miR-184 inhibitor转染至人源SKOV3卵巢癌细胞株中,并采用MTT法对转染目的基因成功的人源SKOV3卵巢癌细胞进行体外增殖活力的检测,同时检测cyclinD1、p27和FOXO3 mRNA的表达水平.结果 卵巢癌组织和细胞中miR-184的表达显著高于癌旁组织,SKOV3细胞…查看全部>>
Objective To investigate the effect of miR-184 on the proliferation of ovarian cancer cells by regula-ting FOXO3.Methods The expression levels of miR-184 in samples were detected using fluorescence quantitative PCR.miR-184 mimic and miR-184 inhibitor were transfected into human SKOV3 ovarian cancer cells.The MTT assay was used to detect the in vitro proliferation activity of successfully transfected human SKOV3 ovarian cancer cells.The ex-pression levels of …查看全部>>
李少儒;户瑞丽;秦海霞
新乡医学院第一附属医院妇科(河南新乡 453100)新乡医学院第一附属医院妇科(河南新乡 453100)新乡医学院第一附属医院妇科(河南新乡 453100)
临床医学
卵巢癌miR-184细胞增殖FOXO3
ovarian neoplasmsmiR-184cell proliferation abilityFOXO3
《广东医学》 2023 (11)
1341-1345,5
河南省医学科技攻关计划联合共建立项项目(LHGJ20200525)
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