广西科学院学报2023，Vol.39Issue(4)：452-460,9.DOI:10.13657/j.cnki.gxkxyxb.20231226.012
几丁质降解菌的筛选及其降解虾壳废弃物的研究
Screening of Chitin-degrading Bacteria and the Study of Their Efficient Degradation of Shrimp Shell Waste
摘要
Abstract
The increasing production of shrimp and crab shell waste has caused huge waste of resources and se-rious environmental pollution.In order to improve the comprehensive utilization value of shrimp and crab shell waste,this study screened chitin-degrading bacteria from soil,and explored the optimal conditions for degrading shrimp shells and the degradation ability of shrimp shells through single factor experiments.Using colloidal chitin as the sole carbon source,the soil microorganisms derived from karst landform was enriched,separated and purified by plate transparent circle method.Four strains with chitin-degrading activity were ob-tained:Serratia marcescens gxas3,Bacillus cereus gxas5,Stenotrophomonas maltophilia gxas7 and Chitini-lyticum aquatile gxas1.Among them,C.aquatile gxas1 reached the maximum crude enzyme activity of 0.244 U/mL at 96 h,which was better than the other three strains.The ability of C.aquatile gxas1 to de-grade shrimp shell waste was investigated by single factor experiment,and its medium and culture conditions were optimized.Finally,the optimal enzyme production medium was determined to be fructose 2 g/L,KH2 PO4 0.7 g/L,K2 HPO4 0.3 g/L,MgSO4·6 H2 O 0.6 g/L,yeast powder 0.5 g/L,beef extract 4 g/L,pep-tone 4 g/L.The optimum fermentation conditions were inoculation amount of 2.0%,p H value of 8.0,tem-perature of 20℃and fermentation time of 9 d.Under the optimal fermentation conditions,the maximum deg-radation rate of C.aquatile gxas1 shrimp shell waste reached 60%,the deproteinization rate was 76.77%,and the chitin recovery rate was 87%.This study provides an important theoretical basis for microbial degradation of shrimp and crab shell waste.关键词
微生物降解/几丁质/虾蟹壳废弃物/几丁质降解菌/水生几丁质菌gxas1Key words
biodegradation/chitin/shrimp and crab shell waste/chitin-degraded bacteria/Chitinilyticum aqua-tile gxas1分类
轻工纺织引用本文复制引用
陈建荣,徐雨茶,阳丽艳,黄艳冰,杨登峰,潘丽霞..几丁质降解菌的筛选及其降解虾壳废弃物的研究[J].广西科学院学报,2023,39(4):452-460,9.基金项目
国家自然科学基金项目(31960203),广西科技基地和人才专项(桂科AD23023007)和广西创新驱动发展专项重大科技创新基地建设项目"非粮生物质酶解国家重点实验室"(2022-36-Z06)资助. （31960203）
