广西科学院学报2023，Vol.39Issue(4)：461-470,10.DOI:10.13657/j.cnki.gxkxyxb.20231226.013
UPLC-QQQ-MS/MS法用于粉防己根双苄基异喹啉生物碱合成途径中12种化合物的定量检测
Quantitative Determination of 12 Compounds in the Biosynthetic Pathway of Bibenzylisoquinoline Alkaloids in the Roots of Stephania tetrandra S.Moore by UPLC-QQQ-MS/MS
摘要
Abstract
It is of great significance for the simultaneous qualitative and quantitative detection of organic acids and alkaloids in Stephania tetrandra S.Moore by Ultra-High Performance Liquid Chromatography-Tandem Triple Quadrupole Mass Spectrometry(UPLC-QQQ-MS/MS)method.It can provide data reference for the analysis of the metabolic synthesis mechanism of bisbenzylisoquinoline alkaloids such as tetrandrine's biosyn-thetic pathway in S.tetrandra S.Moore.In this study,a Poroshell 120 CS-C18 column was used for separa-tion,with 10 mmol/L ammonium formate aqueous solution(A)-acetonitrile(B)containing 0.1%formic acid as the mobile phase for gradient elution,and the detection was carried out under the positive and negative ion Multiple Reaction Monitoring(MRM)mode of Agilent Jet Stream Electrospray Ionization(AJS-ESI).Quali-tative and quantitative analysis of Stephania tetrandra S.Moore root metabolites in different batches was carried out by primary and secondary mass spectrometry data.The results showed that the 12 compounds showed a good linear relationship in the linear range,and the correlation coefficients were all greater than 0.99.In the roots of S.tetrandra S.Moore,there were significant differences in the content of 12 tested com-pounds,and the same compound also had significant differences in different collection months.The contents of L-tyrosine,tetrandrine and fangchinoline in the roots of S.tetrandra S.Moore were relatively high.In this study,the simultaneous qualitative and precise quantitative analysis of 12 compounds in S.tetrandra S.Moore have been realized.关键词
粉防己/超高效液相色谱-三重四极杆串联质谱/双苄基异喹啉生物碱/定性分析/定量分析Key words
Stephania tetrandra S.Moore/ultra-high performance liquid chromatography-tandem triple qua-drupole mass spectrometry/bibenzylisoquinoline alkaloids/qualitative analysis/quantitative analysis分类
化学化工引用本文复制引用
申润艳,陈衍炽,李检秀,蒙丽钧,严兴,李坚斌,谢能中..UPLC-QQQ-MS/MS法用于粉防己根双苄基异喹啉生物碱合成途径中12种化合物的定量检测[J].广西科学院学报,2023,39(4):461-470,10.基金项目
国家自然科学基金项目(32260245),广西科技重大专项(桂科AA2211700),广西重大科技创新基地建设项目(2022-36-Z06-03,2022-36-Z06-07),广西科技基地和人才专项(2021AC19176),广西科学院创新团队启动基金(CQ-D-24215)和广西科学院发展基金项目(2021YFJ1210)资助. （32260245）
