广西医科大学学报2023，Vol.40Issue(12)：1941-1948,8.DOI:10.16190/j.cnki.45-1211/r.2023.12.002
基于SOCS1-TLR2信号通路探讨HIV/结核分枝杆菌合并感染的发病机制
Exploration of the pathogenesis of HIV/Mtb co-infection based on SOCS1-TLR2 signaling pathway
摘要
Abstract
Objective:To investigate the role of suppressor of cytokine signaling 1(SOCS1)-toll-like receptor 2(TLR2)signaling pathway in macrophages in the promotion of Mycobacterium tuberculosis(Mtb)infection by human immunodeficiency virus(HIV).Methods:Peripheral venous blood was collected from HIV-1 mono-infec-tion patients,Mtb mono-infection patients,HIV-1/Mtb co-infection patients and healthy controls,and peripheral blood mononuclear cells(PBMCs)were isolated,and the expression levels of SOCS1,TLR2 and downstream Mtb infection-related factors in mononuclear macrophages in PBMCs were detected by flow cytometry.Based on the macrophage-HIV-Mtb infection model,the role of SOCS1-TLR2 in HIV-promoted Mtb infection was ex-plored.Results:Compared with the healthy control group,the protein expression levels of interleukin(IL)-6,IL-10 were increased in the HIV-1 mono-infection group,Mtb mono-infection group and HIV-1/Mtb co-infection group(P＜0.05)and the protein expression levels of IL-1β,IL-12 and interferon(IFN)-γ were increased in the Mtb mono-infection group(P＜0.05).In vitro cell experiments,compared with the control group,the expression levels of SOCS1 mRNA in the HIV-1 mono-in-fection group,Mtb mono-infection group and HIV-1/Mtb co-infection group were increased(P＜0.05),and the expression levels of TLR2 mRNA in the HIV-1 mono-infection group and HIV-1/Mtb co-infection group were de-creased(P＜0.05).After constructing an infection group with overexpressed SOCS1,compared with the control group,the expression levels of SOCS1 and IL-6 in the HIV-1 mono-infection group and the HIV-1/Mtb co-infec-tion group were increased(P＜0.05)and the expression levels of SOCS1 and IL-6 in the HIV-1/Mtb co-infection group were decreased after the TLR2 agonist was added(P＜0.05).HIV/Mtb co-infection group with overex-pressed SOCS1 had the highest Mtb colony count(P＜0.05).Conclusion:HIV-1 may facilitate Mtb infection by promoting the high expression of inflammatory cytokines IL-6and IL-10in the SOCS1-TLR2 signaling pathway.关键词
结核分枝杆菌/艾滋病/细胞因子信号传导抑制因子1/Toll样受体2Key words
Mycobacterium tuberculosis/acquired immune deficiency syndrome/suppressor of cytokine signa-ling 1/toll-like receptors 2分类
医药卫生引用本文复制引用
林雪,廖艳研,潘沛江,王建,钟伟月,叶力,梁浩..基于SOCS1-TLR2信号通路探讨HIV/结核分枝杆菌合并感染的发病机制[J].广西医科大学学报,2023,40(12):1941-1948,8.基金项目
国家自然科学基金资助项目(No.81560326) （No.81560326）
