广西医科大学学报2023，Vol.40Issue(12)：1949-1955,7.DOI:10.16190/j.cnki.45-1211/r.2023.12.003
白藜芦醇通过巨噬细胞源性外泌体调节肝星状细胞活化对肝纤维化的影响
Effect of resveratrol on liver fibrosis through regulating hepatic stellate cell activation by mac-rophage-derived extracellular vesicles
摘要
Abstract
Objective:To investigate the mechanism of resveratrol(RSV)on the activation of hepatic stellate cells by regulating macrophages.Methods:Exosomes were extracted from blank(NC-E)group and resveratrol-treated(RSV-E)group by ultracentrifugation method,and the extracts were identified.The exosomes from the NC-E group and the RSV-E group were co-cultured with growth factor-β1(TGF-β1)-activated hepatic stellate cells JS-1 and divided into control group,TGF-β1-activated group(TGF-β1 group),NC-E+TGF-β1-activated group(NC-E+TGF-β1 group)and RSV-E+TGF-β1-activated group(RSV-E+TGF-β1 group).Immunofluores-cence,real-time fluorescence quantitative PCR(RT-qPCR)and western blotting were used to detect the alpha-smooth muscle actin(α-SMA)and type I collagen alpha 1 chain(Col1a1).Western blotting was also used to de-tect the expression of autophagy-related indicators Beclin1 and LC3Ⅰ/Ⅱ.Results:Compared with the control group,the TGF-β1 group showed increased expression levels of α-SMA,Col1a1,Beclin1 and LC3Ⅰ/Ⅱ(P＜0.05).Compared with the TGF-β1 group,the RSV-E+TGF-β1 group showed decreased expression levels of α-SMA,Col1a1,Beclin1 and LC3Ⅰ/Ⅱ(P＜0.05),while the NC-E+TGF-β1 group and TGF-β1 group showed no significant difference(P＞0.05).Conclusion:RSV may inhibit the progression of liver fibrosis by regulating macrophage-derived extracellular vesicles and its mechanism may be related to the inhibition of autophagy in he-patic stellate cells.关键词
白藜芦醇/肝纤维化/外泌体/巨噬细胞/肝星状细胞/自噬Key words
resveratrol/liver fibrosis/macrophage-derived extracellular vesicles/macrophages/hepatic stellate cells/autophagy分类
医药卫生引用本文复制引用
梁耀丹,井洁,张泰程,姜海行,覃山羽..白藜芦醇通过巨噬细胞源性外泌体调节肝星状细胞活化对肝纤维化的影响[J].广西医科大学学报,2023,40(12):1949-1955,7.基金项目
国家自然科学基金资助项目(No.81960119) （No.81960119）
广西壮族自治区卫生健康委自筹经费科研课题资助项目(No.Z-A20230546) （No.Z-A20230546）
