广西医科大学学报2023，Vol.40Issue(12)：1985-1989,5.DOI:10.16190/j.cnki.45-1211/r.2023.12.008
槲皮素调控锌稳态抑制脂多糖诱导的小鼠巨噬细胞炎症反应
Quercetin inhibiting lipopolysaccharide-induced inflammation in mouse macrophages by modulating zinc homeostasis
摘要
Abstract
Objective:To investigate the effect and mechanism of quercetin(QUE)on lipopolysaccharide(LPS)-induced inflammatory response in macrophages of mice.Methods:Mouse RAW264.7 macrophages were divid-ed into normal control group,LPS group,and QUE+LPS group.The levels of interleukin(IL)-1β,tumor necrosis factor(TNF)-α and IL-6 in cell culture supernatants were detected by enzyme-linked immunosorbent assay(ELI-SA).The free zinc content in FluoZin-3 labeled cells was detected by flow cytometry,the levels of ZIP8 mRNA expression in each group of cells were detected by real-time fluorescence quantitative PCR(RT-qPCR),and the expression of ZIP8 protein was detected by western blotting.Results:Compared with the LPS group(1 μg/mL),the contents of IL-1β,TNF-α and IL-6 in the macrophage supernatants of the QUE+LPS group were decreased(P＜0.01).Compared with the LPS group,the content of free zinc in macrophages of the QUE+LPS group were decreased(P＜0.05)and the LPS-induced ZIP8 expression were inhibited(P＜0.05).Conclusion:QUE can in-hibit LPS-induced macrophage inflammatory response,and its effect may be related to down-regulating ZIP8 ex-pression,inhibiting zinc influx,and reducing free zinc content in macrophages.关键词
巨噬细胞/锌稳态/槲皮素/炎症反应Key words
macrophages/zinc homeostasis/quercetin/inflammatory response分类
医药卫生引用本文复制引用
韦晓林,陈艳青,黄一丹,杨青萍,毛振宇,董良..槲皮素调控锌稳态抑制脂多糖诱导的小鼠巨噬细胞炎症反应[J].广西医科大学学报,2023,40(12):1985-1989,5.基金项目
国家自然科学基金资助项目(No.82260019) （No.82260019）
柳州市科技计划项目(No.2022CAC0105) （No.2022CAC0105）
广西卫健委自筹课题资助项目(No.Z20210215,Z-B20221367) （No.Z20210215,Z-B20221367）
广西中医药管理局自筹课题资助项目(No.GXZYB20220428) （No.GXZYB20220428）
