广西医科大学学报2023，Vol.40Issue(12)：1997-2003,7.DOI:10.16190/j.cnki.45-1211/r.2023.12.010
大蒜素对COPD大鼠肺组织损伤及内质网应激的影响
Effect of allicin on lung tissue injury and endoplasmic reticulum stress in rats with COPD
摘要
Abstract
Objective:To investigate the effect of allicin(ALL)on lung tissue damage and endoplasmic reticu-lum stress(ERS)in rats with chronic obstructive pulmonary disease(COPD).Methods:Sixty healthy adult male SD rats were randomly divided into normal control(NC)group,model group,allicin low(ALL-L,4 mg/kg),alli-cin medium(ALL-M,8 mg/kg),allicin high(ALL-H,16 mg/kg)dose groups and dexamethasone(DXM,1 mg/kg)group,with 10 rats in each group.The COPD rat models were established by repeated smoke exposure and in-tratracheal injection of lipopolysaccharide(except the rats in NC group).The drugs were given by intraperitoneal injection(the rats in NC group and model group were given physiological saline),once a day for 28 d.The pulmo-nary function indexes[minute ventilation(MV),peak expiratory flow rate(PEF),forced vital capacity(FVC),forced expiratory volume in third second(FEV3)and FEV3/FVC]were measured in each group,the histopatholo-gy changes of lung tissue was observed by HE staining and the apoptosis was observed by TUNEL staining;the contents of IL-1β,IL-6,TNF-α,and IFN-γ in alveolar lavage fluid were detected by ELISA;the expression of ERS related proteins was detected by western blotting.Results:Compared with the model group,the MV,PEF,FVC,FEV3,and FEV3/FVC in the ALL-M,ALL-H groups and DXM group were increased(P＜0.05);the lung tissue pathological changes and apoptosis were significant improved,and the apoptosis index(AI)was decreased(P＜0.05);the contents of IL-1β,IL-6,TNF-α,IFN-γ were decreased(P＜0.05);the relative expression of GRP78,p-PERK,CHOP,NF-κB,Cleaved Caspase-12,Cleaved Caspase-3 were decreased(P＜0.05).Except FEV3/FVC,TNF-α and NF-κB,ALL-L,ALL-M and ALL-H groups showed dose-dependent effects on other indexes(P<0.05).Conclusion:ALL can reduce lung tis-sue damage and improve lung function in COPD rats and the mechanism may be related to inhibiting ERS and its mediated inflammatory response and apoptosis.关键词
慢性阻塞性肺疾病/大蒜素/内质网应激/凋亡Key words
chronic obstructive pulmonary disease/allicin/endoplasmic reticulum stress/apoptosis分类
医药卫生引用本文复制引用
王倩倩,刘莎莎,王丽斌,左瑞静,陈惠敏,阚敏宸..大蒜素对COPD大鼠肺组织损伤及内质网应激的影响[J].广西医科大学学报,2023,40(12):1997-2003,7.基金项目
河北省医学科学研究课题(No.20210110) （No.20210110）
