华北农学报2023，Vol.38Issue(6)：144-155,12.DOI:10.7668/hbnxb.20194362
化肥减量配施腐植酸生物肥对芸豆碳氮代谢及产量的影响
Effects of Fertilizer Reduction Combined with Humic Acid Bio-fertilizer on Carbon and Nitrogen Metabolism and Yield of Kidney Bean
摘要
Abstract
By investigating the effects of chemical fertilizer reduction with humic acid bio-fertilizer on the car-bon and nitrogen metabolic processes of kidney bean leaves,it provides a theoretical basis for the application of hu-mic acid fertilizer for kidney beans in Northeast China.This trial was conducted at the Anda Experimental Base of Bayi Agricultural Reclamation University in Heilongjiang,using the kidney bean variety Baisak as the test material for two years of field trials in 2021 and 2022.There were six treatments:CF(conventional fertilizer application),RF(fertilizer reduction of 20%),RFH1(fertilizer reduction of 20%+45.0 kg/ha humic acid bio-fertilizer),RFH2(fertilizer reduction of 20%+67.5 kg/ha humic acid bio-fertilizer),RFH3(fertilizer reduction of 20%+90.0 kg/ha humic acid bio-fertilizer),RFH4(fertilizer reduction of 20%+112.5 kg/ha humic acid bio-fertilizer).The experiment was conducted to determine the indicators of carbon and nitrogen metabolism and their products throughout the reproduc-tive period of kidney bean,and to determine the yield of kidney bean and its constituent factors at the harvesting stage.The activities of sucrose synthase(SS),sucrose phosphate synthase(SPS),acidic invertase(AI),neutral invertase(NI)in carbon metabolism and nitrate reductase(NR),nitrite reductase(NiR),glutamate synthase(GOGAT)and glutamine syn-thetase(GS)in nitrogen metabolism were analyzed by fertilizer reduction with humic acid bio-fertilizer,and the activities of carbon and nitrogen metabolites sucrose,soluble sugar,starch and soluble protein.To determine the effect of humic acid on the carbon and nitrogen metabolism of kidney bean.The results showed that the application of humic acid bio-fer-tilizer increased the activity of enzymes related to carbon and nitrogen metabolism in kidney bean leaves,increased the content of carbon and nitrogen metabolites and improved the yield of kidney bean compared with CF treatment.During the reproductive period,SS activity of RFH2 treatment was significantly increased by 9.5%—15.8%compared to CF treatment.RFH3 treatment significantly increased the SPS activity by 16.5%—38.1%compared to CF treatment.The AI activity of RFH3 treatment was significantly increased by 10.8%—25.5%compared to CF treatment.RFH2 treatment in-creased NI activity by 14.0%—32.1%.With the application of humic acid bio-fertilizer,NR and NiR activities were sig-nificantly increased by22.6%—50.1%and 16.3%—19.8%,and GOGAT activities were significantly increased by 16.0%—35.7%in the RFH2 treatment compared to the CF treatment.RFH3 treatment significantly increased the GS activity by 14.5%—44.1%compared to CF treatment.Meanwhile,among the treatments with humic acid bio-fertilizer,RFH2 treatment significantly increased the carbon and nitrogen metabolites sucrose,soluble sugar,starch and soluble protein in kidney bean leaves by 7.6%—9.3%,14.1%—26.8%,6.7%—10.5%and 3.7%—8.8%,respectively and the starch content significanthy increased by 0.8-1.3 percentage points.In addition,the applica-tion of humic acid bio-fertilizer was able to increase the yield of kidney bean,with a significant increase of 24.8%(2021)and 21.5%(2022)in the RFH2 treatment compared to the CF treatment.The treatment of 20%reduction in conventional chemical fertilizer application with 67.5 kg/ha of humic acid bio-fertilizer(RFH2)could increase the activity of enzymes related to carbon and nitrogen metabolism in kidney bean leaves,increase the accumulation of carbon and nitrogen metabolites in leaves,and increase the yield of kidney bean.关键词
芸豆/化肥减量/腐植酸生物肥/碳氮代谢Key words
Kidney bean/Fertilizer reduction/Humic acid bio-fertilizer/Carbon-nitrogen metabolism分类
农业科技引用本文复制引用
孔德庸,邢力文,韦娜,王诗雅,孙光旭,刘莹,王欣怡,郭伟,孙海燕..化肥减量配施腐植酸生物肥对芸豆碳氮代谢及产量的影响[J].华北农学报,2023,38(6):144-155,12.基金项目
国家重点研发计划(2020YFD1001402) （2020YFD1001402）
黑龙江八一农垦大学"三纵"基础培育(ZRCPY202101) （ZRCPY202101）
