广西植物2023，Vol.43Issue(12)：2269-2279,11.DOI:10.11931/guihaia.gxzw202210058
外源NO处理对四种桉树幼苗铝胁迫抗性的影响
Effects of exogenous NO treatment on aluminum stress resistance of four Eucalyptus seedlings
摘要
Abstract
To investigate the effect of exogenous nitric oxide(NO)on aluminum tolerance of Eucalyptus seedlings under aluminum stress.In this study,sodium nitroproside(SNP)was used as exogenous NO donor in four kinds of Eucalyptus seedlings(Eucalyptus grandis,E.urophylla,E.tereticornis,E.urophylla×E.grandis).The effects of different concentrations of NO(0,50,100,200,400,800 μmol·L-1)on the physiological indices of ROS,antioxidant enzyme activities and the contents of organic osmotic regulatory substances of Eucalyptus seedlings under 120 mg·L-1 aluminum stress were studied,and the differences of aluminum tolerance of four Eucalyptus species under NO treatment were compared.The results were as follows:(1)The addition of exogenous NO(50 μmol·L-1≤NO≤200 μmol·L-1)under aluminum stress promoted the contents of soluble sugar and soluble protein,the activities of antioxidant enzymes(SOD,POD,CAT,APX),the removal of ROS in the body,the reduction of MDA accumulation,and the improvement of aluminum stress resistance.However,under high concentration of NO(≥800 μmol·L-1),the activities of antioxidant enzymes and the contents of osmotic regulatory substances decreased in Eucalyptus seedlings,showing stress response.(2)NO significantly improved the aluminum tolerance of sensitive Eucalyptus,but not significantly improved the aluminum tolerance of tolerant Eucalyptus.Finally,the aluminum resistance of four Eucalyptus species tended to be consistent under the action of NO.(3)SOD,MDA,CAT,O2-,soluble protein and soluble sugar could be used as key indicators to evaluate the aluminum tolerance of Eucalyptus.This study provides a scientific reference for the selection of aluminum tolerance germplasm resources of Eucalyptus,and laid a foundation for further understanding of the mechanism of NO regulating the difference of aluminum tolerance between Eucalyptus species.关键词
桉树/外源NO/铝胁迫/生理指标/主成分分析/耐铝性Key words
Eucalyptus/exogenous NO/aluminium stress/physiological indexes/principal component analysis/aluminum tolerance分类
生物学引用本文复制引用
刘冰,郭荣琨,石茂鑫,罗义山,蒋丰璟,滕维超..外源NO处理对四种桉树幼苗铝胁迫抗性的影响[J].广西植物,2023,43(12):2269-2279,11.基金项目
国家自然科学基金(31800530) （31800530）
广西自然科学基金(2023GXNSFAA026485,2018GXNSFBA281009). （2023GXNSFAA026485,2018GXNSFBA281009）
