华北农学报2023，Vol.38Issue(6)：192-200,9.DOI:10.7668/hbnxb.20194239
牦牛FTL基因克隆、生物信息学及表达分析
Cloning,Bioinformatics Analysis and Expression Analysis of FTL Gene in Yak
摘要
Abstract
The aim of this study was to understand the structure and function of ferritin light chain(FTL)gene in yak and its expression pattern in eight tissues of different age groups.The coding sequence(CDS)of FTL gene was cloned from the cDNA of liver tissue of adult(36 months old)Meiren yak,and bioinformatics analysis was car-ried out.The relative expression of FTL gene in heart,liver,spleen,lung,kidney,longissimus dorsi,fat and testis was detected by Real-time fluorescence quantitative(qRT-PCR).The results showed that the CDS region of FTL gene of Meiren yak was 528 bp,encoding 175 amino acids,and had the closest relationship with wild yak and cattle(99.8%).FTL protein was a stable hydrophilic secretory protein without signal peptide and transmembrane struc-ture.It was mainly distributed in the cytoplasm.The secondary structure of the protein was mainly α-helix,which mainly interacted with FTH1,SCARA5,NCOA4 and other proteins.The results of qRT-PCR showed that the expression levels of FTL gene in adult cattle were liver,kidney,testis,spleen,lung,fat,longissimus dorsi and heart,respectively.The expression levels of FTL gene in fat and longissimus dorsi tissues of 7-day-old calves were the highest,and the expression level of FTL gene in liver tissue of adult cattle was the highest.By comparing the two stages,it was found that the expres-sion levels of fat,longissimus dorsi and heart tissues decreased with the increase of age,while the expression levels of lung,spleen,testis,kidney and liver tissues increased with age.关键词
美仁牦牛/FTL基因/克隆/生物学分析/组织表达Key words
Meiren yak/FTL/Gene cloning/Biological analysis/Tissue expression分类
农业科技引用本文复制引用
张娟香,郭宪,褚敏,阎萍,梁春年,马小勇,路建卫,仁青吉,罗胜伟,肖光峰,王有鹤,马晓明,喇永福..牦牛FTL基因克隆、生物信息学及表达分析[J].华北农学报,2023,38(6):192-200,9.基金项目
甘肃省科技计划项目(20JR5RA580) （20JR5RA580）
甘肃省科技重大项目(21ZD10NA001 （）
GZGG-2021-1) （）
合作市牦牛种质提升与提质增效项目、现代农业(肉牛牦牛)产业技术体系建设专项资金(CARS-37) （肉牛牦牛）
中国农业科学院科技创新工程(25-LZIHPS-01) （25-LZIHPS-01）
